Investigation of combined effects of xylanase and glucose oxidase in whole wheat buns making based on reconstituted model dough system
文献类型: 外文期刊
第一作者: Yang, Mei
作者: Yang, Mei;Yue, Ying;Liu, Liya;Tong, Litao;Wang, Lili;Ashraf, Jawad;Li, Nana;Zhou, Xianrong;Zhou, Sumei
作者机构:
关键词: Whole wheat dough; Arabinoxylan; Xylanase; Glucose oxidase; Buns
期刊名称:LWT-FOOD SCIENCE AND TECHNOLOGY ( 影响因子:4.952; 五年影响因子:5.383 )
ISSN: 0023-6438
年卷期: 2021 年 135 卷
页码:
收录情况: SCI
摘要: The combined effects of xylanase (XYL) and glucose oxidase (GOX) during whole wheat buns making was investigated based on the reconstituted model dough system including starch, gluten, and water-unextractable solids (WUS). The results showed that the combinations of GOX and XYL significantly (P < 0.05) increased free sulfhydryl (SH) contents (14.93-70.18%) and glutenin macropolymer (GMP) content (2.82%-6.15%) compared with the individual GOX. The XYL-GOX modified dough significantly (P < 0.05) increased glutenin (5.71%-13.04%) and gliadin solubility (3.57%-6.01%), leading to a decrease in the dough viscoelasticity and a more open gluten matrix structure. Additionally, XYL800GOX80 modified dough exhibited the highest alpha-helix (40.22%) and beta-sheet (43.78%) contents among all the samples. However, when the level of XYL increased from 800 mu g/g to 2400 mu g/g, the alpha-helix and beta-sheet in the XYL2400GOX40 and XYL2400GOX80 showed a significant downward trend (alpha-helix: 3.87-7.81%; beta-sheet: 11.38-19.90%; P < 0.05). Therefore, the study indicated that the synergistic effects of XYL and GOX on the whole wheat dough were quite dependent on the hydrolysis degrees of WUS.
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