文献类型： 外文期刊
第一作者： Li, Xuefei
作者： Li, Xuefei;Li, Shiyu;Sossah, Frederick Leo;Han, Xuerong;Li, Yu;Li, Changtian;Fu, Yongping;Liu, Qingcheng;Zhu, Guosheng;Liu, Qingcheng;Zhu, Guosheng;Sossah, Frederick Leo
作者机构：
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.685； 五年影响因子：2.506 ）
ISSN： 0304-8608
年卷期： 2022 年 167 卷 11 期
页码：
收录情况： SCI
摘要： Here, we report the characterization of a novel (-)ssRNA mycovirus isolated from Auricularia heimuer CCMJ1222, using a combination of RNA-seq, reverse transcription polymerase chain reaction, 5 ' and 3 ' rapid amplification of cDNA ends, and Sanger sequencing. Based on database searches, sequence alignment, and phylogenetic analysis, we designated the virus as "Auricularia heimuer negative-stranded RNA virus 1" (AhNsRV1). This virus has a monopartite RNA genome related to mymonaviruses (order Mononegavirales). The AhNsRV1 genome consists of 11,441 nucleotides and contains six open reading frames (ORFs). The largest ORF encodes a putative RNA-dependent RNA polymerase; the other ORFs encode hypothetical proteins with no conserved domains or known function. AhNsRV1 is the first (-)ssRNA virus and the third virus known to infect A. heimuer.
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