Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1

文献类型： 外文期刊

第一作者： Wang, Qingjie

作者： Wang, Qingjie;Zhao, Qiping;Zhu, Shunhai;Huang, Bing;Yu, Shuilan;Liang, Shanshan;Wang, Haixia;Zhao, Huanzhi;Han, Hongyu;Dong, Hui

作者机构：

关键词： Apicomplexa; Eimeria tenella; Apical membrane antigen; iTRAQ; DF-1 cells

期刊名称：PARASITE （ 影响因子：3.0； 五年影响因子：3.046 ）

ISSN： 1252-607X

年卷期： 2020 年 27 卷

页码：

收录情况： SCI

摘要： Apical membrane antigen 1 (AMA1) is a type I integral membrane protein that is highly conserved in apicomplexan parasites. Previous studies have shown that Eimeria tenella AMA1 (EtAMA1) is critical for sporozoite invasion of host cells. Here, we show that EtAMA1 is a microneme protein secreted by sporozoites, confirming previous results. Individual and combined treatment with antibodies of EtAMA1 and its interacting proteins, E. tenella rhoptry neck protein 2 (EtRON2) and Eimeria-specific protein (EtESP), elicited significant anti-invasion effects on the parasite in a concentration-dependent manner. The overexpression of EtAMA1 in DF-1 cells showed a significant increase of sporozoite invasion. Isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS were used to screen differentially expressed proteins (DEPs) in DF-1 cells transiently transfected with EtAMA1. In total, 3953 distinct nonredundant proteins were identi?ed and 163 of these were found to be differentially expressed, including 91 upregulated proteins and 72 downregulated proteins. The DEPs were mainly localized within the cytoplasm and were involved in protein binding and poly(A)-RNA binding. KEEG analyses suggested that the key pathways that the DEPs belonged to included melanogenesis, spliceosomes, tight junctions, and the FoxO and MAPK signaling pathways. The data in this study not only provide a comprehensive dataset for the overall protein changes caused by EtAMA1 expression, but also shed light on EtAMA1's potential molecular mechanisms during Eimeria infections.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序