Isolation and Expression Analysis of OfLIS Gene in Osmanthus fragrans

文献类型： 外文期刊

第一作者： Chen, Rui

作者： Chen, Rui;Hu, Ju;Song, Haiyan;Xian, Xiaolin;Pan, Yuanzhi

作者机构：

关键词： Osmanthus fragrans var. JinyuTaige; Floral scent; Linalool synthase; Expression level; Phosphorylation

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2020 年 24 卷 6 期

页码：

收录情况： SCI

摘要： Osmanthus fragrans Lour., originating from the Yangtze River valley of China, has high ornamental value and is an important source of floral scent materials. Linalool is an important marker of the floral scentin O. fragrans. In this study, a linalool synthase-encoding gene (OfLIS, GenBank No. MK563985) was cloned from O. fragrans var. Jinyu Taige by rapid amplification of cDNA (complementary DNA) ends (RACE). The full-length of OfLIS was 2911 bp in length and consisted of an open reading frame (ORF) of 2523 bp, encoding a protein consisting of 840 amino acid residues. Sequence alignment indicated that OfLIS contains the conserved DDxxD motif and RLxxDxxxxxxExxxG motif. The phylogenetic analysis revealed OfLIS belongs to subfamily II of the dicot group and has the closest relationship with PaLIS. Tissue-specific expression analysis showed that the OfLIS gene is specifically expressed in flowers and its expression is highest at peak flowering stage (PFS). In addition, 17 potential phosphorylation sites were found in OfLIS, and 531Y is located in the DDxxD motif. The cloning and expression analysis of OfLIS gene helps further our understanding of the mechanism of floral scent formation in O. fragrans. (C) 2020 Friends Science Publishers

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