Dynamic Transcriptome Analysis of a Multi-Silique Trait in Rapeseed (Brassica napus)

文献类型： 外文期刊

第一作者： Chai, Liang

作者： Chai, Liang;Zhang, Jinfang;Li, Haojie;Jiang, Jun;Cui, Cheng;Zheng, Benchuan;He, Ping;Zhu, Lijuan;Jiang, Liangcai

作者机构：

关键词： Brassica napus; Multi-silique; Dynamic transcriptome; Differentially expressed gene

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2020 年 24 卷 6 期

页码：

收录情况： SCI

摘要： Flower and fruit development are vital in plant life and aberrance in floral structure results in abnormal fruit morphology. In this study, we investigated molecular mechanism of multi-silique phenomenon in oil crop Brassica napus, using dynamic transcriptome analysis. Plants in multi-silique rapeseed line "zws-ms" and its near isogenic line (NIL) "zws-217" were sampled at flowering and green podding stage. Total RNA was isolated for transcriptome sequencing (RNA-seq). The T07-T12 group (at flowering stage) obtained 62.44 Gb while the T13 similar to T18 group (at podding stage) obtained 56.58 Gb raw data. Samples in the two groups generated 34.9 M and 31.6 M clean reads, respectively, with 71.30 similar to 75.09% of them mapped to reference genome. At the flowering stage, 123 genes varied in expression level, among which 70 were up-regulated in zws-ms and 53 were down-regulated; at the podding stage, the number of differentially expressed genes raised to 268, with 219 of them up-regulated and 49 down-regulated in zws-ms. Further analysis found that 77 DEGs (differentially expressed genes) shared the same expression tendencies at the two stages. Sixty out of the 77 DEGs got either Gene Ontology annotation or the Kyoto Encyclopedia of Genes or Genomes pathway information, and 56 of them had corresponding orthologs in model plant Arabidopsis. The findings of dynamic transcriptome analysis furthered our understanding about molecular mechanism of multi-silique trait in Brassica napus. (C) 2020 Friends Science Publishers

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