Effects of dietary arginine levels on growth, intestinal peptide and amino acid transporters, and gene expressions of the TOR signaling pathway in tiger puffer, Takifugu rubripes

文献类型： 外文期刊

第一作者： Wei, Yuliang

作者： Wei, Yuliang;Zhang, Qinggong;Jia, Linlin;Xu, Houguo;Liang, Mengqing;Wei, Yuliang;Xu, Houguo;Liang, Mengqing

作者机构：

关键词： Tiger puffer; Arginine; Growth performance; Intestinal peptide and amino acid transporters; TOR signaling pathway

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2021 年 532 卷

页码：

收录情况： SCI

摘要： This study aimed to determine the dietary arginine requirement of tiger puffer (Takifugu rubripes), and to assess the effect of dietary arginine levels on intestinal peptide and amino acid transporters, and target of rapamycin (TOR) signaling pathway in muscle. Six experimental diets were formulated to contain 480 g kg 1 crude protein from fishmeal, corn protein concentrate and crystalline amino acid premix with graded levels of arginine (19.1, 21.5, 24.4, 26.7, 30.0 and 32.5 g kg(-1) of dry diet). Each diet was randomly fed to triplicate groups of 25 fish (an average initial body weight of 19.97 g) per tank. Final body weight, specific growth rate (SGR), feed efficiency ratio, protein efficiency ratio and protein productive value increased with the increase of dietary arginine levels from 19.1 to 24.4 g kg(-1) (p < 0.05), and then showed a declining trend although there was no significant difference. With the increase of dietary arginine levels, intestinal oligopeptide transporter 1 (PepT1), cationic amino acid transporter 1 (CAT1) and y(+) L-type amino acid transporter 2 (y(+)LAT2) mRNA levels increased at first and thereafter showed a decreasing trend, where a break point was observed in the 24.4 g kg(-1) arginine group. The expression of b(0,+)-type amino acid transporter (b(0,+) AT) in intestine was significantly higher in the 21.5 and 24.4 g kg(-1) arginine groups compared with the 32.5 g kg(-1) arginine group (p < 0.05). Gene expressions of TOR, ribosomal protein S6 kinase 1 and eukaryotic translation initiation factor 4E-binding protein 1 in muscle were not statistically different in all treatment groups (p > 0.05). In conclusion, an optimal level of dietary arginine stimulated intestinal peptide and amino acid transporters, while gene expressions of TOR signaling pathway in muscle were not responsive to dietary arginine levels. Based on nonlinear regression analysis of SGR, the dietary arginine requirement of tiger puffer was estimated to be 24.40 g kg(-1) of dry diet (50.12 g kg(-1) of dietary protein).

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序