Full-length transcriptome sequencing and modular organization analysis of oleanolic acid- and dammarane-type saponins related gene expression patterns in Panax japonicus

文献类型： 外文期刊

第一作者： Xu, Ran

作者： Xu, Ran;Zhang, Jiao;Zhang, Shaopeng;Zhu, Wenjun;Xiong, Chao;Chen, Ping;Liu, Zhiguo;You, Jingmao;Guo, Jie;Gao, Limei;Xiong, Hui;Li, Yongchang;Shu, Shaohua

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关键词： Panax japonicus; Saponin distribution; Biosynthesis; Rhizoma and root tissues; Metabolome; Full-length transcriptome

期刊名称：GENOMICS （ 影响因子：5.736； 五年影响因子：4.939 ）

ISSN： 0888-7543

年卷期： 2020 年 112 卷 6 期

页码：

收录情况： SCI

摘要： The saponins found in Panax japonicus, a traditional medicinal herb in Asia, exhibit high degrees of structural and functional similarity. In this study, metabolite analysis revealed that oleanolic acid-type and dammaranetype saponins were distributed unevenly in three tissues (rhizome_Y, rhizome_O, and secRoot) of P. japonicus. Single-molecule real-time (SMRT) sequencing and next generation sequencing (NGS) data revealed distinct and tissue-specific transcriptomic patterns relating to the production of these two types of saponins. In the co -expression network and hierarchical clustering analyses, one 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and two 1-deoxy-D-xylulose-5-phosphate synthase (DXS) etc. transcripts were found to be key genes associated with the biosynthesis of oleanolic acid and dammarane-type saponins in P. japonicus, respectively. In addition, cytochrome p450 (CYP) and UDP-glucuronosyltransferase (UGT) family proteins that serve as regulators of saponin biosynthesis-related genes were also found to exhibit tissue-specific expression patterns. Together these results offer a comprehensive metabolomic and transcriptomic overview of P. japonicus.

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