Immune and physiological responses of juvenile Chinese sea bass (Lateolabrax maculatus) to eugenol and tricaine methanesulfonate (MS-222) in gills

文献类型: 外文期刊

第一作者: Wang, Wenhao

作者: Wang, Wenhao;Dong, Hongbiao;Sun, Yongxu;Sun, Caiyun;Duan, Yafei;Xie, Mujiao;Zhang, Jiasong;Wang, Wenhao;Sun, Caiyun;Gu, Qunhong;Li, Yong;Zhang, Jiasong

作者机构:

关键词: Tricaine methanesulfonate; Eugenol; Gill; Immunity; Stress

期刊名称:AQUACULTURE REPORTS ( 影响因子:3.216; 五年影响因子:3.812 )

ISSN: 2352-5134

年卷期: 2020 年 18 卷

页码:

收录情况: SCI

摘要: This study investigates the impact of two types of anesthetics, tricaine methanesulfonate (MS-222) and eugenol, in the gills of juvenile Lateolabrax maculatus. Fish (Mean +/- SD, 98.1 +/- 7.3 g) were randomly exposed to three treatments, MS-222 (50 mg/L), eugenol (10 mg/L), or anesthetic-free water (control), each with five replicates. Gills were sampled at 0, 1, 3, 6, 12, 24, and 48 h after exposure to the above anesthetics for 20 min. MS-222 and eugenol both had significant immunomodulatory effects on gills. Physiological assays revealed that MS-222 and eugenol induced apoptosis and oxidative stress and changed the morphology of the gills as well as the Na+-K+ flux crossing the gill membrane. These results suggest that immune and physiological responses can be used to evaluate the negative impact of anesthetics on gills.

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