Construction of a recombinant vaccinia virus expressing Babesia gibsoni thrombospondin-related anonymous protein and evaluation of its immunogenicity in mice
文献类型： 外文期刊
第一作者： Nakamura, C.
作者： Nakamura, C.;Liu, M. M.;Goo, Y. K.;Zhang, G. H.;Jia, H. L.;Kumagai, A.;Zhang, H. S.;Zhou, J. L.;Nishikawa, Y.;Xuan, X. N.;Goo, Y. K.;Jia, H. L.;Zhang, H. S.;Zhou, J. L.
作者机构： Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan;Kyungpook Natl Univ, Dept Parasitol & Trop Med, Sch Med, Daegu 700422, South Korea;Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150069, Peoples R China;Chinese Acad Agr Sci, Shanghai Vet Res Inst, Minist Agr, Key Lab Anim Parasitol, Shanghai 200241, Peoples R China
期刊名称：TROPICAL BIOMEDICINE （ 2020影响因子：0.623； 五年影响因子：0.697 ）
ISSN： 0127-5720
年卷期： 2020 年 37 卷 4 期
收录情况： SCI
摘要： Previously, we have identified a gene encoding thrombospondin-related anonymous protein of Babesia gibsoni (BgTRAP), and have shown that the antisera raised against recombinant BgTRAP expressed in Escherichia coli inhibited the growth of parasites. In the present study, a recombinant vaccinia virus expressing the BgTRAP (VV/BgTRAP) was constructed. A specific band with a molecular mass of 80 kDa, which is similar to that of native BgTRAP on the merozoites of B. gibsoni, was detected in the supernatant of VV/BgTRAP-infected RK13 cells. Mice inoculated with VV/BgTRAP produced a specific anti-BgTRAP response. The antiserum against VV/BgTRAP showed reactivity against the native BgTRAP on parasites. These results indicated that the recombinant vaccinia virus expressing BgTRAP might be a vaccine candidate against canine B. gibsoni infection.
- 相关文献
作者其他论文 更多>>
-
Taxonomy, phylogeny and divergence times of Polyporus (Basidiomycota) and related genera
作者：Ji, X.;Zhou, J. L.;Song, C. G.;Xu, T. M.;Cui, B. K.;Wu, D. M.;Zhou, J. L.
关键词：molecular clock; morphology; multi-gene phylogenies -; new species; Polyporaceae
-
Molecular detection and genotyping of Anaplasma spp. and Theileria spp. infections in sheep and cattle from the northeast region of China
作者：Wang, G. B.;Moumouni, Adjou P. F.;Efstratiou, A.;Xuan, X. N.;Yang, C.;Tong, J. J.;Ren, X. L.;Liu, Y.;Chang, J. T.;Cao, S. N.;Zhou, M.
关键词：
-
Cloning and Functional Analysis of TaHSP83 Involved in the Defence Response Against Puccinia Triticina in TcLr19
作者：Hu, Y. Y.;Zhang, N.;Zhang, H. S.;Li, J. Y.;Yang, W. X.;Liu, D. Q.;Hu, Y. Y.
关键词：wheat leaf rust;TaHSP83;rapid amplification cDNA ends (RACE);virus induced gene silencing (VIGS);subcellular localization
-
Regulation of ATG6/Beclin-1 homologs by abiotic stresses and hormones in rice (Oryza sativa L.)
作者：Rana, R. M.;Dong, S.;Huang, J.;Zhang, H. S.;Rana, R. M.;Ali, Z.;Ali, Z.
关键词：Autophagy;Abiotic stress;Phylogeny;Rice
-
Effect of several supplemental Chinese herbs additives on rumen fermentation, antioxidant function and nutrient digestibility in sheep
作者：Qiao, G. H.;Zhou, X. H.;Li, Y.;Zhang, H. S.;Li, J. H.;Wang, C. M.;Lu, Y.
关键词：Chinese herbs;rumen fermentation;nutrient digestibility;antioxidant function;sheep
-
GENETIC RELATIONSHIPS OF CHINESE GRAPE ACCESSION TO EUROPEAN AND AMERICAN CULTIVARS ASSESSED BY MICROSATELLITE MARKERS
作者：Guo, D. L.;Zhang, J. Y.;Zhang, G. H.;Li, M.;Liu, C. H.
关键词：grape;microsatellite;genetic relationship;UPGMA;PCoA
-
Chromosomal Mapping and QTL Analysis of Resistance to Downy Mildew in Cucumis sativus
作者：Zhang, S. P.;Liu, M. M.;Miao, H.;Gu, X. F.;Zhang, S. Q.;Wehner, T. C.
关键词：
