Molecular quantification of spider preying on springtails in winter-spring fallow waterlogged paddy fields

文献类型： 外文期刊

第一作者： Sun, Jiaqi

作者： Sun, Jiaqi;Pang, Shuai;Bai, Yaoyu;Wang, Xueqin;Wang, Guanghua;Wang, Xueqin;Wang, Guanghua;Zhu, Zengrong;Cheng, Jiaan

作者机构：

关键词： Winter-spring fallow waterlogged paddy fields; Springtail; Spider predation; Molecular gut analysis; Real-time quantitative PCR

期刊名称：APPLIED ENTOMOLOGY AND ZOOLOGY （ 影响因子：1.403； 五年影响因子：1.381 ）

ISSN： 0003-6862

年卷期： 2021 年 56 卷 2 期

页码：

收录情况： SCI

摘要： Fallow waterlogged paddy field (FWPF) is a subtropical type of winter-spring fallow rice fields. Arthropod predation is crucial for their survival during the long-duration FWPF seasons. No research has been conducted in the trophic link of arthropod predators and their prey in FWPFs. Here, we used a quantitative real-time PCR approach, both in lab and field, to detect the trophic link. DNA-based identification confirmed the results of a morphological classification in two dominant species of springtail, Homidia socia Denis and H. tiantaiensis Chen and Lin (Collembola: Entomobryidae). The fastest degradation in H. socia DNA copy numbers in spider Pirata subpiraticus (Bosenberg and Strand) (Araneida: Lycosidae) guts occurred within 8 h under lab conditions. Quantitative comparisons of preying on H. socia by H. socia DNA copy numbers indicated that Oedothorax insecticeps (Bosenberg and Strand), Erigonidium graminicolum (Sundevall) (Araneida: Linyphiidae) and P. subpiraticus were more active springtail predators than others under field conditions. There were significant differences in the DNA copy numbers of springtails in gut of P. subpiraticus among different overwintering periods that was significantly positive related to the springtail densities. This study highlights springtail importance at species level as the prey of spiders in FWPFs and illustrates the utility of our approach to quantify their trophic linkages among different spider species or postharvest seasons.

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