Fabrication of chondroitin sulfate calcium complex and its chondrocyte proliferation in vitro

文献类型： 外文期刊

第一作者： Shen, Qingshan

作者： Shen, Qingshan;Zhang, Chunhui;Zhang, Hongru;Qin, Xiaojie;Li, Juan;Shen, Qingshan;Zhang, Hongru;Richel, Aurore;Mo, Haizhen;Zhang, Zhiqiang

作者机构：

关键词： Chondroitin sulfate; Calcium; Chondrocyte; Proliferation; Bone care

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

ISSN： 0144-8617

年卷期： 2021 年 254 卷

页码：

收录情况： SCI

摘要： Chondroitin sulfate (CS)-calcium complex (CSCa) was fabricated, and the structural characteristics of CSCa and its proliferative bioactivity to the chondrocyte were investigated in vitro. Results suggested calcium ions could bind CS chains forming polysaccharide-metal complex, and the maximum calcium holding capacity of CSCa reached 4.23 %. Characterization of CSCa was performed by EDS, AFM, FTIR, UV, XRD and 1H-NMR. It was found that calcium ions were integrated with CS by binding the sulfate or carboxyl groups. The thermal properties analysis indicated CSCa had a good thermal stability by TGA and DSC. CSCa could interact the calcium-sensing receptor increasing the intracellular calcium ions and influence the cell cycle. The TGF-beta 1 secretion induced by CSCa could activate the TGF-beta/Smads pathway and change the genes associated proliferation expression ultimately leading to the chondrocyte proliferation. This research probably has an important implication for understanding the effect of CSCa on bone care as food supplements.

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