In vitro and In vivo Antibacterial Effects of Nisin Against Streptococcus suis

文献类型： 外文期刊

第一作者： Zhu, Haodan

作者： Zhu, Haodan;Han, Lixiao;Ni, Yanxiu;Yu, Zhengyu;Wang, Dandan;Zhou, Junming;Li, Bin;He, Kongwang;Zhu, Haodan;Ni, Yanxiu;Yu, Zhengyu;Wang, Dandan;Zhou, Junming;Li, Bin;He, Kongwang;Zhu, Haodan;Ni, Yanxiu;Yu, Zhengyu;Wang, Dandan;Zhou, Junming;Li, Bin;He, Kongwang;Han, Lixiao;Zhang, Wei

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关键词： Nisin; Streptococcus suis; MIC; MBC; Antibiofilm; Balb/c mouse

期刊名称：PROBIOTICS AND ANTIMICROBIAL PROTEINS （ 影响因子：4.609； 五年影响因子：4.585 ）

ISSN： 1867-1306

年卷期： 2021 年 13 卷 2 期

页码：

收录情况： SCI

摘要： Nisin is a promising therapeutic candidate because of its potent activity against Gram-positive bacteria. The present study aimed to describe the in vitro and in vivo antibacterial effects of nisin against Streptococcus suis, an important zoonotic pathogen. The minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) of nisin against different S. suis strains ranged from 0.12 to 4.0 mu g/mL and from 0.25 to 8.0 mu g/mL, respectively. Time-killing curve assays illustrated that nisin killed 100% of tested virulent S. suis strains within 4 h when used at 2x MIC, which indicates the rapid bactericidal activity of nisin against the bacteria. Transmission and scanning electron microscopy revealed that nisin destroyed S. suis cell membrane integrity and affected its cellular ultrastructure, including a significantly wrinkled surface, intracellular content leakage, and cell lysis. In addition, nisin inhibited biofilm formation by S. suis in a concentration-dependent manner and exhibited strong degrading activities against preformed biofilms. More importantly, nisin displayed antimicrobial activity against S. suis infection in vivo. Upon treatment with 5.0-10 mg/kg nisin solution, the survival rates of mice challenged with a lethal dose of virulent S. suis virulent ranged 87.5-100%. Nisin significantly decreased bacterial proliferation and translocation in the mouse spleen, brain, and blood. These results indicate that nisin has potential as a novel antimicrobial agent for the clinical treatment and prevention of infection caused by S. suis in animals.

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