NtRBP45, a nuclear RNA-binding protein of Nicotiana tabacum, facilitates post-transcriptional gene silencing

文献类型： 外文期刊

第一作者： Zhang, Wangbin

作者： Zhang, Wangbin;Zhu, Zongcai;Yan, Hailin;Zhang, Wangbin;Zhu, Zongcai;Du, Peixiu;Zhang, Chao;Yan, Hailin;Li, Weimin;Wang, Wenguo

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关键词： antiviral defense; post-transcriptional gene silencing; RNA binding protein; siRNA accumulation

期刊名称：PLANT DIRECT （ 影响因子：3.038； 五年影响因子：3.017 ）

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年卷期： 2020 年 4 卷 12 期

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收录情况： SCI

摘要： The tobacco RBP45 is a nuclear RNA binding protein (RBP). In this study, we identified that the gene expression of NtRBP45 was significantly up-regulated upon the Tobacco mosaic virus infection and the central region of the protein accounted for its nuclear localization. In particular, using a green fluorescent protein-based transient suppression assay, we uncovered that the transiently overexpressed NtRBP45 was able to enhance local post-transcriptional gene silencing (PTGS), facilitate siRNA accumulation, and compromise the RNA silencing suppression mediated by Tomato aspermy virus 2b protein. Deletion mutagenesis showed that both the N- and C-terminal regions of NtRBP45 were necessary for enhancing PTGS. The data overall indicated a novel RNA silencing factor that might participate in antiviral defense.

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