Antioxidant and Immunomodulatory Properties of Partially purified Exopolysaccharide from Lactobacillus Casei Isolated from Chinese Northeast Sauerkraut

文献类型： 外文期刊

第一作者： Xu, Xiaoqing

作者： Xu, Xiaoqing;Qiao, Yu;Peng, Qing;Shi, Bo;Xu, Xiaoqing;Dia, Vermont P.

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关键词： Exopolysaccharide; Lactobacillus casei NA-2; radical-scavenging; RAW 264; 7 macrophages; cytokines; reactive oxygen species (ROS)

期刊名称：IMMUNOLOGICAL INVESTIGATIONS （ 影响因子：3.657； 五年影响因子：3.279 ）

ISSN： 0882-0139

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收录情况： SCI

摘要： Background: Exopolysaccharides (EPS) from Lactobacillus spp. have been found to have biological activities. Our previous work demonstrated the antibiofilm activity of EPS from Lactobacillus casei NA-2 (L.casei NA-2) isolated from northeast Chinese sauerkraut (Suan Cai). The present study has focussed on the antioxidant and immunomodulatory activities of the EPS in vitro. Methods: Antioxidant properties of the EPS were evaluated by the radical-scavenging activities in vitro. The immunomodulatory effects of EPS were assayed by measuring nitric oxide (NO), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and reactive oxygen species (ROS) in RAW 264.7 macrophages, and the mechanism was investigated through NF-kappa B and JNK. Result: EPS contains 88% total sugar, with the molecular weights (Mw) of 1.3 x 10(6) Da, 6.4 x 10(5) Da, 2.0 x 10(5) Da, and 1.4 x 10(4) Da. EPS showed antioxidant activity by scavenging hydroxyl radicals (42% at 1.2 mg/mL), superoxide radicals (76% at 100 mu g/mL), and DPPH (80% at 10 mg/mL); and did not affect the proliferation of unstimulated or lipopolysaccharide (LPS)-induced RAW 264.7 cells at the concentrations ranging from 31.25 to 500 mu g/mL. Results showed EPS promoted the production of ROS and TNF-alpha involved in NF-kappa B p65 and JNK signaling pathways in unstimulated RAW 264.7 cells. On the other hand, the levels of NO and iNOS were reduced after EPS treatment in LPS-induced RAW 264.7 cells. Conclusion: Our results showed the protective effect against oxidative damage and potential immunomodulatory and anti-inflammatory properties of EPS from Lactobacillus casei NA-2.

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