Identification of a 1,274-bp promoter of a Musa acuminata L. aquaporin gene (MaPIP1;1) which confers salinity stress inducibility in transgenic Arabidopsis
文献类型: 外文期刊
第一作者: Song, Shun
作者: Song, Shun;Ma, Chaozhi;Li, Jingyang;Wang, Wenquan;Song, Shun;Ma, Funing;Huang, Dongmei;Wu, Bin;Li, Jingyang;Li, Yujia;Wei, Qing;Sun, Qiurong;Xu, Yi;Ma, Funing;Xu, Yi
作者机构:
期刊名称:AGRONOMY JOURNAL ( 影响因子:2.24; 五年影响因子:2.829 )
ISSN: 0002-1962
年卷期: 2021 年 113 卷 2 期
页码:
收录情况: SCI
摘要: Salt stress will seriously influence alt stress in banana (Musa L.) induces MaPIP1;1, which enhances the salt resistance of transgenic Arabidopsis. The promoter pMaPIP1;1, located 13,62 bp upstream of the transcriptional start site, was isolated from the banana genome, and four pMaPIP1;1::GUS fusion constructs namely M-P1, M-P2, M-P3, and M-P4 were used to transform into Arabidopsis. In this work, in order to functionally verify the promoter responds to high salt stress, we used NaCl treatment for simulating salt terms. NaCl treatment is responded to by four transformants. M-P2 (-1,274 bp to -1) showed the highest transcriptional activity among all transgenic Arabidopsis transformants. This area of the promoter endows high glucuronidase (GUS) enzyme activity according to NaCl treatment. These results will help us understand the transcriptional regulatory of MaPIP1;1, and promoter fragment M-P2 will be an ideal candidate for overexpression of salt response genes to increase plant resistance.
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