Biochemical characterization of a GH19 chitinase from Streptomyces alfalfae and its applications in crystalline chitin conversion and biocontrol

文献类型： 外文期刊

第一作者： Lv, Chenyin

作者： Lv, Chenyin;Gu, Tianyan;Yao, Wei;Zhao, Guogang;Ma, Rui;Huang, Yuyang;Gu, Jingang

作者机构：

关键词： GH19 chitinase; Enzymatic conversion; Anti-fungi

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 167 卷

页码：

收录情况： SCI

摘要： Chitinases play crucial roles in enzymatic conversion of chitin and biocontrol of phytopathogenic fungi. Herein, a chitinase of glycoside hydrolase (GH) family 19, SaChiB, was cloned from Streptomyces alfalfae ACCC 40021 and expressed in Escherichia coli BL21(DE3). The purified SaChiB displayedmaximal activities at 45 degrees C and pH 8.0, and showed good stability up to 55 degrees C and in the range of pH 4.0-11.0, respectively. It exhibited substrate specificity towards chitin and chitooligosaccharides (degree of polymerization 3-6) with the endo-cleavage manner. In combination with the N-acetyl hexosaminidase SaHEX, it yielded a conversion rate of 95.2% from chitin powder to N-acetyl-D-glucosamine in 8 h and a product purity of >98.5%. Furthermore, the enzyme strongly inhibited the growth of tested pathogenic fungi. These results indicated that SaChiB has the great potential for applications in the conversion of raw chitinous waste in industries as well as the biocontrol of fungal diseases in agriculture. (C) 2020 Published by Elsevier B.V.

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