Identification of novel antioxidant peptides from snakehead (Channa argus) soup generated during gastrointestinal digestion and insights into the anti-oxidation mechanisms

文献类型： 外文期刊

第一作者： Zhang, Jin

作者： Zhang, Jin;Zhang, Gaonan;Xiong, Shanbai;Zhao, Siming;Du, Hongying;Zhang, Jin;Kong, Fanbin;Li, Mei;Li, Mei;Tian, Yu;Xiong, Shanbai;Zhao, Siming;Du, Hongying;Jia, Dan;Manyande, Anne

作者机构：

关键词： Antioxidant peptides; Snakehead soup; Gastrointestinal digestion; Molecular docking; UPLC-MS/MS; RP-HPLC; Kelch-like ECH-associated protein 1; Cytoprotective effect

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2021 年 337 卷

页码：

收录情况： SCI

摘要： Antioxidant peptides obtained from snakehead (Channa argus) soup (SHS) after simulated gastrointestinal (GI) digestion were separated, identified and characterized. Results showed that the fraction with MW < 3 kDa had the highest antioxidant capacity. Four novel antioxidant peptides were identified after RP-HPLC and UPLC-MS/MS. PGMLGGSPPGLLGGSPP and SDGSNIHFPN had the highest DPPH radical scavenging activity (IC50 = 1.39 mM) and Fe2+ chelating ability (IC50 = 4.60 mM), respectively. Structures in silico for IVLPDEGK, PGMLGGSPPGLLGGSPP and SDGSNIHFPN suggest at least one beta-turn and/or alpha-helix, which are associated with antioxidant activity. Moreover, our results showed that these three peptides docked with a recombinant Kelch-like ECH-associated protein 1 (Keap1) with a binding score greater than TX6, a good ligand of Keap1. The cell viability assay also showed significant cytoprotective effects against H2O2-induced cellular oxidative damage. This information implies that antioxidant mechanisms of novel SHS peptides occurred via activation of cellular anti-oxidation Keap1-Nrf2 signaling pathway.

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