Identification and genetic analysis of two maize CMS-T mutants obtained from out-space-flighted seeds
文献类型： 外文期刊
第一作者： Yi, Hongyang
作者： Yi, Hongyang;Zhang, Caibo;Li, Chuan;Wang, Jing;Yu, Tao;Liu, Yongming;Cao, Moju;Zhang, Caibo;Liu, Yongming
作者机构：
关键词： Maize; CMS-T; Fertility restoration pattern; PCR amplification; Cytological observation; AFLP analysis
期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：1.6； 五年影响因子：1.7 ）
ISSN： 0925-9864
年卷期： 2021 年 68 卷 5 期
页码：
收录情况： SCI
摘要： Male sterility is widely utilized for hybrid seed production. In this study, two new found male sterile mutants SauS4 and SauS5 were obtained from space flighted seeds of maize inbred line RP125. Then, genetic analysis, molecular markers identification, and cytological observation were conducted to confirm their male sterile types. For genetic analysis, the above two male sterile mutants were continuously backcrossed with two maize inbred line 18Hong and RP125, and four stable male sterile lines SauS4(18Hong), SauS5(18Hong), SauS4(RP125), SauS5(RP125) were generated by six-generation backcross. Restoring and maintaining relationship analysis showed that both Hui313 and Zifeng1 didn't rescue the male sterility SauS4(18Hong) and SauS5(18Hong). Using CMS mitochondria-specific primers for PCR detection suggested that only a 440 bp band unique to CMS-T type was amplified in SauS4(18Hong), SauS5(18Hong), SauS4(RP125), and SauS5(RP125). Sequencing results showed that these bands sequences were identical in DNA level which compared with T-urf13. Cytological observations showed that the main abortion stages of SauS4 and SauS5 were at the middle stage of uninucleate microspores under the two nuclear backgrounds of 18Hong and RP125, exhibiting the characteristics of sporophyte sterility. All the above results pointed out the two male sterile mutants SauS4 and SauS5 belonged to the CMS-T type. Interestingly, some mitochondrial genome difference between SauS4(RP125) and SauS5(RP125) were revealed by AFLP analysis.
分类号：
- 相关文献
作者其他论文 更多>>
-
Heavy rainfall stimulates more N2O emissions from wheat fields during basal and overwintering fertilization phases
作者：Wang, Jing;Liu, Qiang;Liu, Qiang;Zhao, Suya;He, Shiying;Yang, Bei;Wang, Ning;Hou, Pengfu;Xue, Lihong;Yang, Linzhang;Zhao, Suya;He, Shiying;Wang, Ning;Hou, Pengfu;Xue, Lihong
关键词：Rainfall; Nitrous oxide; Nitrogen transformation; Functional microorganisms
-
Soil bacterial diversity and community structure of Suaeda glauca vegetation in the Hetao Irrigation District, Inner Mongolia, China
作者：Dong, Ruixiao;Wang, Xinbo;Li, Yuyi;Zhang, Hongyuan;Li, Xiaobin;Song, Jiashen;Chang, Fangdi;Feng, Wenhao;Pang, Huancheng;Wang, Jing
关键词：Suaeda glauca; saline-alkali soil; rhizosphere; bacteria; community structure
-
Identification of candidate gene associated with maize northern leaf blight resistance in a multi-parent population
作者：Bi, Yaqi;Jiang, Fuyan;Yin, Xingfu;Shaw, Ranjan K.;Guo, Ruijia;Wang, Jing;Fan, Xingming
关键词：Zea mays; Northern leaf blight; Multi-parent population; Genome-wide association analysis; QTL mapping; CATETO germplasm
-
Metabolomics, volatolomics, and bioinformatics analyses of the effects of ultra-high pressure pretreatment on taste and flavour parameters of cured Culter alburnus
作者：Liu, Dongyin;Zhou, Mingzhu;Tan, Hongyuan;Xiong, Guangquan;Wang, Lan;Shi, Liu;Wu, Wenjin;Qiao, Yu;Liu, Dongyin;Zhou, Mingzhu;Tan, Hongyuan;Xiong, Guangquan;Wang, Lan;Shi, Liu;Wu, Wenjin;Qiao, Yu;Liu, Dongyin;Li, Chuan;Zhou, Mingzhu;Tan, Hongyuan
关键词：Ultra -high pressure pretreatment; Fish curing processing; Microorganism; Cathepsin; Metabolism; Volatile and non-volatile compounds
-
Full-length transcriptome and RNA-Seq analyses reveal the resistance mechanism of sesame in response to Corynespora cassiicola
作者：Jia, Min;Ni, Yunxia;Zhao, Hui;Liu, Xintao;Yan, Wenqing;Zhao, Xinbei;Wang, Jing;He, Bipo;Liu, Hongyan;Jia, Min;Ni, Yunxia;Zhao, Hui;Liu, Hongyan
关键词：Sesame; full-length transcriptome; WGCNA; resistant; susceptible; Corynespora cassiicola
-
Inhibition of Hyperglycemia and Hyperlipidemia by Blocking Toll-like Receptor 4: Comparison of Wild-Type and Toll-like Receptor 4 Gene Knockout Mice on Obesity and Diabetes Modeling
作者：Zhao, Xingyu;Huang, Wuyang;Zhao, Xingyu;Zheng, Jiawei;Huang, Wuyang;Wang, Jing;Huang, Wuyang;Li, Bin
关键词：TLR4; glycolipid metabolism; obesity and diabetes modeling; knockout
-
Genome-wide characterization of the CPA gene family in potato and a preliminary functional analysis of its role in NaCl tolerance
作者：Liu, Jintao;Li, Dianjue;Wang, Jing;Wang, Qian;Fu, Qi;Zhu, Guangtao;Liu, Jintao;Zhu, Guangtao;Guo, Xiao;Yang, Xiaohui;Kear, Philip
关键词：Potato; CPA; NaCl stress; Gene expression; Ion transporter
