Identification and genetic analysis of two maize CMS-T mutants obtained from out-space-flighted seeds

文献类型： 外文期刊

第一作者： Yi, Hongyang

作者： Yi, Hongyang;Zhang, Caibo;Li, Chuan;Wang, Jing;Yu, Tao;Liu, Yongming;Cao, Moju;Zhang, Caibo;Liu, Yongming

作者机构：

关键词： Maize; CMS-T; Fertility restoration pattern; PCR amplification; Cytological observation; AFLP analysis

期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：1.6； 五年影响因子：1.7 ）

ISSN： 0925-9864

年卷期： 2021 年 68 卷 5 期

页码：

收录情况： SCI

摘要： Male sterility is widely utilized for hybrid seed production. In this study, two new found male sterile mutants SauS4 and SauS5 were obtained from space flighted seeds of maize inbred line RP125. Then, genetic analysis, molecular markers identification, and cytological observation were conducted to confirm their male sterile types. For genetic analysis, the above two male sterile mutants were continuously backcrossed with two maize inbred line 18Hong and RP125, and four stable male sterile lines SauS4(18Hong), SauS5(18Hong), SauS4(RP125), SauS5(RP125) were generated by six-generation backcross. Restoring and maintaining relationship analysis showed that both Hui313 and Zifeng1 didn't rescue the male sterility SauS4(18Hong) and SauS5(18Hong). Using CMS mitochondria-specific primers for PCR detection suggested that only a 440 bp band unique to CMS-T type was amplified in SauS4(18Hong), SauS5(18Hong), SauS4(RP125), and SauS5(RP125). Sequencing results showed that these bands sequences were identical in DNA level which compared with T-urf13. Cytological observations showed that the main abortion stages of SauS4 and SauS5 were at the middle stage of uninucleate microspores under the two nuclear backgrounds of 18Hong and RP125, exhibiting the characteristics of sporophyte sterility. All the above results pointed out the two male sterile mutants SauS4 and SauS5 belonged to the CMS-T type. Interestingly, some mitochondrial genome difference between SauS4(RP125) and SauS5(RP125) were revealed by AFLP analysis.

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