Differential proteomics reveals main determinants for the improved pectinase activity in UV-mutagenized Aspergillus niger strain

文献类型： 外文期刊

第一作者： Lin, Weiling

作者： Lin, Weiling;Lv, Ruirui;Huang, Wei;ul Haq, Hafeez;Gao, Yuanyuan;Ren, Hongli;Lan, Canhua;Tian, Baoyu;Lin, Weiling;Xu, Xiaohong;Huang, Wei

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关键词： Aspergillus niger mutant; Differential proteomics; Key determinants; Pectin-degrading enzymes; Synergy

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

ISSN： 0141-5492

年卷期： 2021 年 43 卷 4 期

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收录情况： SCI

摘要： Objectives To reveal the potential mechanism and key determinants that contributed to the improved pectinase activity in Aspergillus niger mutant EIMU2, which was previously obtained by UV-mutagenesis from the wild-type A. niger EIM-6. Results Proteomic analysis for Aspergillus niger EIMU2 by two-dimensional electrophoresis demonstrated that mutant EIMU2 harbored a multiple enzyme system for the degradation of pectin, mainly constituting by main-chain-cleaving enzymes polygalacturonase, pectate lyase, pectinesterase, and some accessory enzymes rhamnogalacturonan lyase and arabinofuranosidase. Further quantitatively differential proteomic analysis revealed that the quantities of four proteins, pectinesterase, rhamnogalacturonan lyase A, DNA-directed RNA polymerase A, and a hypothetical protein in strain EIMU2 were much higher than those in EIM-6. PCR amplification, sequencing and alignment analysis of genes for the two main members of pectin-degrading enzymes, pectate lyase and polygalacturonase showed that their sequences were completely consistent in A. niger EIM-6 and mutant EIMU2. Conclusions The result demonstrated that the improved pectinase activity by UV-mutagenesis in A. niger EIMU2 was probably contributed to the up-regulated expression of rhamnogalacturonan lyase, or pectinesterase, which resulted in the optimization of synergy amongst different components of pectin-degrading enzymes.

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