PEGylated nano-Rehmannia glutinosa polysaccharide induces potent adaptive immunity against Bordetella bronchiseptica

文献类型： 外文期刊

第一作者： Huang, Yee

作者： Huang, Yee;Xiao, Chenwen;Su, Fei;Li, Ke;Ji, Quan-An;Wei, Qiang;Liu, Yan;Bao, Guolian;Nan, Li

作者机构：

关键词： Rehmannia glutinosa polysaccharide; PEGylation; Bordetella bronchiseptica; Dendritic cells; Adaptive immunity

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 168 卷

页码：

收录情况： SCI

摘要： Vaccines, in many cases, stimulate only too weak immunogenicity to prevent infection. Therefore, adjuvants are required during their preparation to boost the immune response. We herein developed a PEGylated nanoadjuvant based on Rehmannia glutinosa polysaccharide (RGP). The addition of PEG layer exhibits enhanced immune performance of the nano-RGP. Stimulation of dendritic cells (DCs) with PEGylated nano-RGP (pRL) led to increased proliferation and cytokine production (IL-6, IL-12, IL-1 beta and TNF-alpha). The pRL was internalized into DCs via a rapid and efficient method. The mice immunized with pRL exhibited enhanced antigen-specific serum IgG and Th1-(IFN-gamma), Th2-(IL-4), and Th17-( IL-17, IL-6) cytokine production, contributing to a good anti-infection performance. Furthermore, the pRL could effectively deliver the antigen to the lymph nodes (LNs), activate DC in the LN and produce enhanced CD4(+) and CD8(+) T-cells-derived memory (CD44(high) CD62L(high)), and effector (CD44(high) CD62L(low)) as well as functional phenotypes. Our results revealed that pRL can act as a promising adjuvant with targeted delivery of antigen due to its effective activation and robust adaptive immunity induction of DCs. (c) 2020 Elsevier B.V. All rights reserved.

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