Molecular characterization and expression analysis of double-stranded RNA-dependent protein kinase (PKR) in Dabry's sturgeon (Acipenser dabryanus)

文献类型： 外文期刊

第一作者： Guo, Huizhi

作者： Guo, Huizhi;Yuan, Hanwen;Deng, Dan;Han, Panpan;Li, Qian;Xu, Qiaoqing;Chen, Dunxue;Yuan, Hanwen;Wei, Qiwei

作者机构：

关键词： Acipenser dabryanus; antiviral; expression; PKR; poly I; C stimulation

期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）

ISSN： 0175-8659

年卷期： 2021 年 37 卷 2 期

页码：

收录情况： SCI

摘要： Protein kinase RNA-activated (PKR) is an important component of the innate immune system induced by interferon. This antiviral protein senses a variety of stresses and pathogen-driven double-stranded RNAs (dsRNAs). In the present study, we cloned and characterized the PKR gene from Dabry's sturgeon (Acipenser dabryanus). Bioinformatics analysis revealed that the AdPKR gene comprises a coding sequence (CDS) of 1848 bp, encoding a 616 amino acids (aa) polypeptide. AdPKR shares high aa identity/similarity with proteins from other fish, ranging from 33.4%/51.8% to 87.6%/91.2%, and relatively low aa identity (13.9%) and similarity (26.8%) with human PKR. The AdPKR polypeptide has two dsRNA-binding motifs at the N-terminus and a conserved catalytic kinase domain at the C-terminus. The sturgeon PKR gene contains 17 exons split by 16 introns. Phylogenetic analysis clustered AdPKR together with its counterparts from other teleost fishes. Real-time fluorescene quantitative PCR (RT-qPCR) transcription profiles revealed highest AdPKR expression levels in brain, followed by middle kidney, skin, muscle and head kidney. AdPKR mRNA transcripts were significantly increased at 6 hr post-stimulation (hps) with polyinosinic:polycytidylic acid (poly I:C), remained high at 24 hps, then decreased to normal levels at 48 hps. These results indicate that AdPKR might play a vital role in antiviral immune responses.

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