A versatile biosensing platform coupling CRISPR-Cas12a and aptamers for detection of diverse analytes

文献类型： 外文期刊

第一作者： Zhao, Xiangxiang

作者： Zhao, Xiangxiang;Liu, Guang;Yang, Zhiheng;Zhang, Quanwei;Liang, Mindong;Zhu, Guoliang;Wang, Xinye;Jiang, Lan;Wang, Weishan;Tan, Gao-Yi;Zhang, Lixin;Zhao, Xiangxiang;Liu, Guang;Yang, Zhiheng;Zhang, Quanwei;Liang, Mindong;Zhu, Guoliang;Wang, Xinye;Jiang, Lan;Wang, Weishan;Tan, Gao-Yi;Zhang, Lixin;Li, Shanshan;Wang, Zhong;Zhang, Quanwei;Li, Zilong;Wang, Weishan;Wang, Zhong;Liu, Jiakun;Tong, Yaojun;Wang, Weishan

作者机构：

关键词： CRISPR-Cas12a; Aptamer; Biosensing platform; Diverse analyte; Alpha fetoprotein detection; Cocaine detection

期刊名称：SCIENCE BULLETIN （ 影响因子：11.78； 五年影响因子：7.658 ）

ISSN： 2095-9273

年卷期： 2021 年 66 卷 1 期

页码：

收录情况： SCI

摘要： Rapid and sensitive detection of various analytes is in high demand. Apart from its application in genome editing, CRISPR-Cas also shows promises in nucleic acid detection applications. To further exploit the potential of CRISPR-Cas for detection of diverse analytes, we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR-Cas12a. We demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background, i.e., the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 mu mol/L, respectively, highlighting the advantages of simplicity, sensitivity, short detection time, and low cost compared with the state-of-the-art biosensing approaches. Altogether, this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes. (C) 2020 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.

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