Steptomyces fagopyri sp. nov., a novel actinomycete isolated from rhizospheric soil of Fagopyrum dibotrys

文献类型： 外文期刊

第一作者： Guo, Yihui

作者： Guo, Yihui;Tang, Xinke;Hu, Siren;Li, Kaiqin;Jian, Gao;Zhou, Meiliang;Jian, Gao

作者机构：

关键词： polyphasic taxonomy; rhizosphere soil; Streptomyces fagopyri sp. nov.

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2020 年 70 卷 12 期

页码：

收录情况： SCI

摘要： A novel actinomycete, designated strain QMT-28(T), was isolated from rhizosphere soil of Fagopyrum dibotrys collected from Shuangfeng, Hunan Province, PR China. Strain QMT-28(T) grew well on International Streptomyces Project series media and formed well-developed, branched substrate hyphae and aerial mycelium that differentiated into loose spiral spore chains consisting of cylindrical spores with smooth surfaces. The diagnostic diamino acid was LL-diaminopimelic acid and the whole-cell sugars were galactose and glucose. The predominant fatty acids were C(18:1)cis9, summed feature 6 (C(18:2)cis 9,12/C-18:0 a) and C-16:0. The polar lipids included diphosphatidylglycerol, hydroxy phospatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, phospholipids of unknown structure containing glucosamine and several unidentified phospholipids. The major menaquinones were MK-9, MK-9(H-2), MK-9(H-4), MK-9(H-6) and MK-9(H-8). The genome size of strain QMT-28(T) was about 8.7 Mbp with a G+C content of 71.2 mol%. Phylogenetic analysis showed that the novel strain was closely related to Streptomyces olivochromogenes DSM 40451(T) (99.5% similarity), Streptomyces mirabilis NBRC 13450(T) (98.9%), Streptomyces kanamyceticus NBRC 13414(T) (98.9%), Streptomyces kaempferi I37(T) (98.9%) and Streptomyces arcticus ZLN234(T) (98.8 %). However, the average nucleotide identity values, the digital DNA-DNA hybridization values and the multilocus sequence analysis evolutionary distances between this strain and closely related strains showed that it belonged to a distinct species. In addition, these results were also supported by differences in the phenotypic characteristics between QMT-28(T) and five closely related type strains. Consequently, strain QMT-28(T) should represent a novel species of the genus Streptomyces, with the suggested name Streptomyces fagopyri sp. nov. The type strain is QMT-28(T) (=CICC 24808(T)=JCM 33796(T)).

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