Biological detoxification of fumonisin by a novel carboxylesterase from Sphingomonadales bacterium and its biochemical characterization

文献类型： 外文期刊

第一作者： Li, Zhongyuan

作者： Li, Zhongyuan;Wang, Yan;Liu, Zhongqi;Jin, Shanzheng;Pan, Kungang;Liu, Huihui;Liu, Tianhui;Zhang, Chaozheng;Luo, Xuegang;Song, Yajian;Zhang, Tongcun;Li, Zhongyuan;Wang, Yan;Liu, Zhongqi;Jin, Shanzheng;Pan, Kungang;Liu, Huihui;Liu, Tianhui;Zhang, Chaozheng;Luo, Xuegang;Song, Yajian;Zhang, Tongcun;Li, Xiumei;Zhao, Junqi

作者机构：

关键词： Fumonisin; Carboxylesterase; Biological detoxification

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 169 卷

页码：

收录情况： SCI

摘要： Fumonisins have posed hazardous threat to human and animal health worldwide. Enzymatic degradation is a desirable detoxification approach but is severely hindered by serious shortage of detoxification enzymes. After mining enzymes by bioinformatics analysis, a novel carboxylesterase FumDSB from Sphingomonadales bacterium was expressed in Escherichia coli, and confirmed to catalyze fumonisin B1 to produce hydrolyzed fumonisin B1 by liquid chromatography mass spectrometry for the first time. FumDSB showed high sequence novelty, sharing only similar to 34% sequence identity with three reported fumonisin detoxification carboxylesterases. Besides, FumDSB displayed its high degrading activity at 30-40 degrees C within a broad pH range from 6.0 to 9.0, which is perfectly suitable to be used in animal physiological condition. It also exhibited excellent pH stability and moderate thermostability. This study provides a FB1 detoxification carboxylesterase which could be further used as a potential food and feed additive. (C) 2020 Published by Elsevier B.V.

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