m(6)A mRNA methylation analysis provides novel insights into heat stress responses in the liver tissue of sheep

文献类型： 外文期刊

第一作者： Lu, Zengkui

作者： Lu, Zengkui;Jin, Meilin;Chu, Mingxing;Wei, Caihong;Lu, Zengkui;Liu, Jianbin;Yuan, Chao;Quan, Kai

作者机构：

关键词： Sheep; Heat stress; N-6-methyladenosine; Liver; MeRIP-Seq

期刊名称：GENOMICS （ 影响因子：5.736； 五年影响因子：4.939 ）

ISSN： 0888-7543

年卷期： 2021 年 113 卷 1 期

页码：

收录情况： SCI

摘要： N-6-methyladenosine (m(6)A) mRNA methylation varies in response to stress. However, no map of m(6)A mRNA methylation has been obtained for sheep, nor is it known what effect this has on regulating heat stress in sheep. Here, we obtained m(6)A methylation maps of sheep liver tissues with and without heat stress by MeRIP-seq. In total, 8306 m(6)A peaks associated with 2697 genes were detected in the heat stress group, and 12,958 m(6)A peaks associated with 5494 genes were detected in the control group. Peaks were mainly enriched in coding regions and near stop codons with classical RRACH motifs. Methylation levels of heat stress and control sheep were higher near stop codons, although methylation was significantly lower in heat stress sheep. GO and KEGG revealed that differential m(6)A-containing genes were significantly enriched in the stress response and fat metabolism. Our results showed that m(6)A mRNA methylation modifications regulate heat stress in sheep.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序