Liquefaction of porcine hoof shell to prepare peptone substitute by instant catapult steam explosion

文献类型： 外文期刊

第一作者： Shen, Qingshan

作者： Shen, Qingshan;Wang, Hang;Zhang, Chunhui;Qin, Xiaojie;Jia, Wei;Xu, Xiong;Shen, Qingshan;Richel, Aurore;Zheng, Qiankun

作者机构：

关键词： Instant catapult steam explosion; Porcine hoof shells; Liquefaction; Peptone substitutes; Animal by-products management

期刊名称：JOURNAL OF BIOSCIENCE AND BIOENGINEERING （ 影响因子：2.894； 五年影响因子：2.746 ）

ISSN： 1389-1723

年卷期： 2020 年 129 卷 4 期

页码：

收录情况： SCI

摘要： Instant catapult steam explosion (ICSE) was proposed as a method to liquefy porcine hoof shell (PHS) and prepare a peptone substitute for fermentation culture, achieving environmentally friendly animal by-product recycling. The liquefaction of PHS was conducted at various pressures (0.5-2.3 MPa) for 5-30 min. As evidenced by the scanning electron microscopy analysis, ICSE caused randomly cracks changing the morphological structure of the solid fraction, and ultimately led to protein migration from the solid to liquid phase. Moreover, the chromatographic analysis revealed that the main constituents of the liquid fraction were short peptides (<2 kDa, 84.72%) and amino acids (1.68 mg/mL) at the pressure of 2.3 MPa for 30 min. Subsequently, liquid fractions were prepared as a PHS peptone substitute for fermentation culture. Results suggested the PHS peptone substitute as the main nitrogen source in media was more suitable for the growth of fungus. Therefore, ICSE provides a possibility of large-scale environmentally sustainable management of animal by-products through liquefaction. (C) 2019, The Society for Biotechnology, Japan. All rights reserved.

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