Knockdown of igf3 gene by RNA interference causes gonadal development inhibition of common carp (Cyprinus carpio)
文献类型： 外文期刊
第一作者： Song, Feibiao
作者： Song, Feibiao;Dong, Zaijie;Wang, Lanmei;Zhu, Wenbin;Fu, Jianjun;Dong, Zaijie;Yu, Zhenzhen;Dong, Zaijie
作者机构：
关键词： common carp; dsRNA; gonadal development; igf3; RNAi
期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）
ISSN： 1355-557X
年卷期：
页码：
收录情况： SCI
摘要： RNA interference is an important method in studying the gene function of organisms. As a novel important gene in gonadal development of teleost fish, the function of igf3 is worth of researching. In this study, the igf3b-dsRNA of C. carpio was successfully synthesized, and long-term igf3 interference experiments were conducted. Intraperitoneal injection of igf3b-dsRNA could influence the serum sex hormone level and significantly reduced the expression level of insulin-like growth factors (IGFs) in hypothalamic-pituitary-gonad (HPG) axis. After a 60-day interference experiment, igf3 knockdown could affect the development of ovary and testis of juvenile C. carpio. These findings show that igf3 knockdown may have a negative effect on gonadal development of C. carpio through HPG axis.
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