文献类型： 外文期刊
第一作者： Ma, Yongkai
作者： Ma, Yongkai;Wu, Yanyan;Ma, Yongkai;Wu, Yanyan;Wu, Yanyan;Ma, Yongkai;Huang, Kehui
作者机构：
关键词： P. fucata; antioxidant peptide; cellular antioxidant activity; anti-proliferation; molecular docking
期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）
ISSN： 1017-7825
年卷期： 2021 年 31 卷 1 期
页码：
收录情况： SCI
摘要： Due to the potential of antioxidants to scavenge free radicals in human body, it is important to be able to prepare antioxidant peptides that meet the industrial requirements for cosmetics and food. Here, we determined in vivo/in vitro activities of antioxidant peptide from P. fucata (PFAOP) prepared by bio-fermentation method. The antioxidant property test results showed the DPPH, hydroxyl, superoxide radical-scavenging, and cellular antioxidant activity. EC50 values of PFAOPs were 0.018 +/- 0.005, 0.126 +/- 0.008, 0.168 +/- 0.005, and 0.105 +/- 0.005 mg/ml, respectively, exhibiting higher antioxidant activities than glutathione (p < 0.05). Moreover, anti-proliferation and cytotoxicity activity results illustrated PFAOP has a potent anti-proliferative activity against HepG2, Caco-2, and MCF-7 carcinoma cells with no cytotoxicity. Moreover, the protocols we developed in this work demonstrated several excellent advantages in PFAOP preparation compared to enzymatic hydrolysis or chemical synthesis methods and provide a theoretical foundation for higher-value application of marine-derived functional peptides.
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