Protein kinase PpCIPK1 modulates plant salt tolerance in Physcomitrella patens

文献类型： 外文期刊

第一作者： Xiao, Fei

作者： Xiao, Fei;Li, Xiaochuan;He, Jiaxian;Wu, Guochun;Gong, Qianyuan;Zhou, Huapeng;Lin, Honghui;Zhao, Jinfeng

作者机构：

关键词： Physcomitrella patens (p; patens); Plant salt tolerance; SOS pathway; Calcineurin B-like proteins (CBLs); CBL-interacting protein kinases (CIPKs)

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN： 0167-4412

年卷期： 2021 年 105 卷 6 期

页码：

收录情况： SCI

摘要： Key message This work demonstrates that PpCIPK1, a putative protein kinase, participates in regulating plant salt tolerance in moss Physcomitrella patens. Calcineurin B-Like protein (CBL)-interacting protein kinases (CIPKs) have been reported to be involved in multiple signaling networks and function in plant growth and stress responses, however, their biological functions in non-seed plants have not been well characterized. In this study, we report that PpCIPK1, a putative protein kinase, participates in regulating plant salt tolerance in moss Physcomitrella patens (P. patens). Phylogenetic analysis revealed that PpCIPK1 shared high similarity with its homologs in higher plants. PpCIPK1 transcription level was induced upon salt stress in P. patens. Using homologous recombination, we constructed PpCIPK1 knockout mutant lines (PpCIPK1 KO). Salt sensitivity analysis showed that independent PpCIPK1 KO plants exhibited severe growth inhibition and developmental deficiency of gametophytes under salt stress condition compared to that of wild-type P. patens (WT). Consistently, ionic homeostasis was disrupted in plants due to PpCIPK1 deletion, and high level of H2O2 was accumulated in PpCIPK1 KO than that in WT. Furthermore, PpCIPK1 functions in regulating photosynthetic activity in response to salt stress. Interestingly, we observed that PpCIPK1 could completely rescue the salt-sensitive phenotype of sos2-1 to WT level in Arabidopsis, indicating that AtSOS2 and PpCIPK1 are functionally conserved. In conclusion, our work provides evidence that PpCIPK1 participates in salt tolerance regulation in P. patens.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序