Mass spectrometry-based proteomic analysis of potential infectious bursal disease virus VP3-interacting proteins in chicken embryo fibroblasts cells

文献类型： 外文期刊

第一作者： Ma, Sun-Ting

作者： Ma, Sun-Ting;Wang, Yong-Shan;Wang, Xiao-Li;Xia, Xing-Xia;Bi, Zhen-Wei;Wang, Jing-Yu;Zhu, Yu-Mei;Ouyang, Wei;Qian, Jing;Ma, Sun-Ting;Wang, Yong-Shan;Wang, Xiao-Li;Xia, Xing-Xia;Bi, Zhen-Wei;Wang, Jing-Yu;Zhu, Yu-Mei;Ouyang, Wei;Qian, Jing

作者机构：

关键词： Infectious bursal disease virus; VP3 protein; Host protein factor; Immunoprecipitation technique; Mass spectrometry

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2021 年 57 卷 2 期

页码：

收录情况： SCI

摘要： The structural protein VP3 of infectious bursal disease virus (IBDV) plays a critical role in viral assembly, replication, immune escape, and anti-apoptosis. Interaction between VP3 and host protein factors can affect stages in the viral replication cycle. In this study, 137 host proteins interacting with VP3 protein were screened through liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics approach. The functions and relevance of the proteins were obtained through bioinformatics analysis. Most VP3-interacting proteins were linked to binding, catalytic activity, and structural molecular activity, and performed functions in cell parts and cells. Biological functions of VP3-interacting proteins were mainly relevant to "Cytoskeleton", "Translation", and "Signal transduction mechanisms", involving ribosomes, "Tight junction", regulation of actin cytoskeleton, and other pathways. Six potential VP3-interacting proteins in host cells were knocked down, and vimentin, myosin-9, and annexin A2 were found to be related to IBDV replication. This study would help explore regulatory pathways and cellular mechanisms in IBDV-infected cells, and also provided clues for the in-depth study of VP3 biological functions and IBDV replication or pathogenesis.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序