Metagenomic analysis reveals significant differences in microbiome and metabolic profiles in the rumen of sheep fed low N diet with increased urea supplementation

文献类型： 外文期刊

第一作者： Li, Zhipeng

作者： Li, Zhipeng;Shen, Junshi;Xu, Yixuan;Zhu, Weiyun;Li, Zhipeng;Shen, Junshi;Zhu, Weiyun

作者机构：

关键词： metagenome; rumen microbiome; urea; glutamate; pyruvate; butyrate

期刊名称：FEMS MICROBIOLOGY ECOLOGY （ 影响因子：4.194； 五年影响因子：5.137 ）

ISSN： 0168-6496

年卷期： 2020 年 96 卷 10 期

页码：

收录情况： SCI

摘要： Urea is a cost-effective replacement for feed proteins in ruminant diets. However, its metabolism by the rumen microbiome is not fully understood. Here, rumen contents were collected from 18 male sheep fed one of the following three treatments: a low N basal diet with no urea (UC, 0 g/kg dry matter (DM)), low urea (LU, 10 g/kg DM) and high urea (HU, 30 g/kg DM). Principal coordinate analysis showed that the microbial composition and functional profiles of the LU treatment significantly differed from the UC and HU treatments. The genera Prevotella, Succinivibrio, Succinatimonas and Megasphaera were higher in the LU rumen, while the genera Clostridium, Ruminococcus and Butyrivibrio were enriched in the UC and HU rumen. The aspartate-glutamate and arginine-proline metabolic pathways and valine, leucine and isoleucine biosynthesis were higher in the LU rumen. The cysteine and methionine metabolism, lysine degradation and fructose and pentose phosphate metabolism pathways were higher in the UC and HU rumen. The protozoa population in the HU treatment was higher than in the UC and LU treatments. These findings suggest that the rumen microbiome of sheep fed low N diet with different urea supplementation are significantly different.

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