Microglia Elimination Increases Neural Circuit Connectivity and Activity in Adult Mouse Cortex

文献类型： 外文期刊

第一作者： Liu, Yong-Jun

作者： Liu, Yong-Jun;Tang, Bryan;Xu, Xiangmin;Liu, Yong-Jun;Spangenberg, Elizabeth E.;Green, Kim N.;Spangenberg, Elizabeth E.;Green, Kim N.;Xu, Xiangmin;Holmes, Todd C.;Holmes, Todd C.;Xu, Xiangmin

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关键词： excitatory; perineuronal nets; plasticity; parvalbumin; synaptic connections; visual cortex

期刊名称：JOURNAL OF NEUROSCIENCE （ 影响因子：6.167； 五年影响因子：6.993 ）

ISSN： 0270-6474

年卷期： 2021 年 41 卷 6 期

页码：

收录情况： SCI

摘要： Microglia have crucial roles in sculpting synapses and maintaining neural circuits during development. To test the hypothesis that microglia continue to regulate neural circuit connectivity in adult brain, we have investigated the effects of chronic microglial depletion, via CSF1R inhibition, on synaptic connectivity in the visual cortex in adult mice of both sexes. We find that the absence of microglia dramatically increases both excitatory and inhibitory synaptic connections to excitatory cortical neurons assessed with functional circuit mapping experiments in acutely prepared adult brain slices. Microglia depletion leads to increased densities and intensities of perineuronal nets. Furthermore, in vivo calcium imaging across large populations of visual cortical neurons reveals enhanced neural activities of both excitatory neurons and parvalbumin-expressing interneurons in the visual cortex following microglia depletion. These changes recover following adult microglia repopulation. In summary, our new results demonstrate a prominent role of microglia in sculpting neuronal circuit connectivity and regulating subsequent functional activity in adult cortex.

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