Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach

文献类型： 外文期刊

第一作者： Zeng, Huanchao

作者： Zeng, Huanchao;Qin, Yiru;Du, Erxia;Wei, Qiulan;Li, Ying;Huang, Danyan;Li, Sheng;Li, Na;Zeng, Huanchao;Qin, Yiru;Du, Erxia;Li, Sheng;Li, Na;Wang, Guirong;Veenstra, Jan A.

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关键词： liquid chromatography; mass spectrometry; peptidomics; genomics; neuropeptides; tissue mapping

期刊名称：JOURNAL OF PROTEOME RESEARCH （ 影响因子：4.466； 五年影响因子：4.352 ）

ISSN： 1535-3893

年卷期： 2021 年 20 卷 2 期

页码：

收录情况： SCI

摘要： As a model hemimetabolous insect species and an invasive urban pest that is globally distributed, the American cockroach, Periplaneta americana, is of great interest in both basic and applied research. Previous studies on P. americana neuropeptide identification have been based on biochemical isolation and molecular cloning. In the present study, an integrated approach of genomics- and peptidomics-based discovery was performed for neuropeptide identification in this insect species. First, 67 conserved neuropeptide or neurohormone precursor genes were predicted via an in silico analysis of the P. americana genome and transcriptome. Using a large-scale peptidomic analysis of peptide extracts from four different tissues (the central nervous system, corpora cardiac and corpora allata complex, midgut, and male accessory gland), 35 conserved (predicted) neuropeptides and a potential (novel) neuropeptide were then identified. Subsequent experiments revealed the tissue distribution, sex difference, and developmental patterns of two conserved neuropeptides (allatostatin B and short neuropeptide F) and a novel neuropeptide (PaOGS36577). Our study shows a comprehensive neuropeptidome and detailed spatiotemporal distribution patterns, providing a solid basis for future functional studies of neuropeptides in the American cockroach (data are available via ProteomeXchange with identifier PXD021660).

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