Isolation and Characterization of Bioactive Proteoglycan-Lipid Nanoparticles from Freshwater Clam (Corbicula fluminea Muller) Soup

文献类型： 外文期刊

第一作者： Gao, Guanzhen

作者： Gao, Guanzhen;Wang, Huiqin;Zhou, Jianwu;Rao, Pingfan;Ke, Lijing;Zhang, Yue;Lin, Jing Jen;Pan, Bonnie Sun;Wang, Qiang

作者机构：

关键词： incidental food nanoparticles; proteoglycan-lipid self-assemblies; ion-exchange chromatography; phytosterol; Corbicula fluminea Muller; hepatoprotective

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2021 年 69 卷 5 期

页码：

收录情况： SCI

摘要： Nanoparticles can be prepared by several sophisticated processes but until now, it cannot be prepared by simple home cooking. Here, we report that two incidental food nanoparticles (iFNPs) consisting of proteoglycans and phytosterols were isolated from soup made from freshwater clam (Corbicula fluminea Muller), a renowned folk remedy for liver problems in China and other parts of East Asia. These two bioactive iFNPs were obtained and characterized by anionic exchange chromatography coupled with multi-angle laser light scattering measurement. Their hydrodynamic diameters and zeta-potentials were 50 +/- 0.2 nm and -28.0 mV and 67 +/- 0.4 nm and -9.96 mV, respectively. FT-IR revealed that the proteoglycans in the particles contained alpha-type heteropolysaccharides. Both iFNPs were resistant to pH changes and separation by mechanical force but responsive to temperature changes. They effectively inhibited cholesterol uptake in vitro, which resonates with the traditional belief that freshwater clam soup provides hepatoprotective benefits. This study suggests that these two proteoglycan-lipid iFNPs are the active moieties and offers a supramolecular structure-based approach to study the function of such complex matrices derived from food.

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