Proteome-wide analyses reveal diverse functions of acetylation proteins in Neurospora crassa

文献类型： 外文期刊

第一作者： Wang, Tielin

作者： Wang, Tielin;Du, Yongxi;Xu, Yang;Zhang, Yan;Kang, Chuanzhi;Wan, Xiufu;Chi, Xiulian;Sun, Kai;Zhang, Xiaobo;Guan, Wei;He, Zhen

作者机构：

关键词： acetylated proteins; Neurospora crassa; protein‐ protein interaction; quantitative acetyl‐ proteomics

期刊名称：PROTEOMICS （ 影响因子：3.984； 五年影响因子：3.831 ）

ISSN： 1615-9853

年卷期： 2021 年 21 卷 6 期

页码：

收录情况： SCI

摘要： Quantitative acetyl-proteomics, a newly identified post-translational modification, is known to regulate transcriptional activity in different organisms. Neurospora crassa is a model ascomycete fungus maintained for biochemistry and molecular biology research; however, extensive studies of the functions of its acylation proteins have yet to be performed. In this study, using LC-MS/MS qualitative proteomics strategies, we identified 1909 modification sites on 940 proteins in N. crassa and analysed the functions of these proteins using GO enrichment, KEGG pathway, and subcellular location experiments. We classified the acetylation protein involvement in diverse pathways, and protein-protein interaction (PPI) network analysis further demonstrated that these proteins participate in diverse biological processes. In summary, our study comprehensively profiles the crosstalk of modified sites, and PPI among these proteins may form a complex network with both similar and distinct regulatory mechanisms, providing improved understanding of their biological functions in N. crassa.

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