Identification of viral particles in the apoplast of Nicotiana benthamiana leaves infected by potato virus X

文献类型： 外文期刊

第一作者： Hu, Shuzhen

作者： Hu, Shuzhen;Chen, Jianping;Hu, Shuzhen;Yin, Yueyan;Chen, Buyang;Lin, Qi;Tian, Yanzhen;Peng, Jiejun;Zheng, Hongying;Rao, Shaofei;Wu, Guanwei;Yan, Fei;Chen, Jianping;Lu, Yuwen;Song, Xijiao;Mo, Xiaohan

作者机构：

关键词： apoplast; coat protein; exosomes; potato virus X; viral particles

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）

ISSN： 1464-6722

年卷期： 2021 年 22 卷 4 期

页码：

收录情况： SCI

摘要： The apoplast is the extracellular space for signalling, nutrient transport, and plant-microbe interactions, but little is known about how plant viruses use the foliar apoplast. Proteomic analysis of the apoplasts isolated from potato virus X (PVX)-infected Nicotiana benthamiana plants showed that the coat protein (CP) is the dominant viral component. The presence of the CP in the apoplast was confirmed by western blot, viral nucleic acid was detected by reverse transcription-PCR and northern blot, and viral particles were observed by transmission electron microscopy (TEM). The apoplast from infected leaves was infectious if rubbed onto healthy leaves but not when infiltrated into them. The exosomes were separated from the apoplast fluid by high-speed centrifugation and TEM showed that PVX particles were not associated with the exosomes. These results suggest that PVX virions are released to the N. benthamiana apoplast in a one-way manner and do not share the bidirectional transport of exosomes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序