Herbidospora solisilvae sp. nov., a novel cellulose-degrading actinobacterium isolated from forest soil

文献类型： 外文期刊

第一作者： Yu, Mingying

作者： Yu, Mingying;Zhou, Rui;Li, Jingjing;Han, Liyuan;Wang, Han;Zhang, Siyu;Zhao, Junwei;Wang, Xiangjing;Song, Jia;Xiang, Wensheng;Xiang, Wensheng

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关键词： Herbidospora solisilvae sp; nov; Polyphasic taxonomy; 16S rRNA gene; Forest soil

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.271； 五年影响因子：2.707 ）

ISSN： 0003-6072

年卷期： 2021 年 114 卷 5 期

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收录情况： SCI

摘要： An aerobic, non-motile, Gram-stain positive, cellulose-degrading actinobacterium, designated strain NEAU-GS84(T), was isolated from a forest soil sample collected from Linchun Ridge Forest Park in Sanya, Hainan Province, China, and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics indicated that strain NEAU-GS84(T) could belong to the genus Herbidospora. The 16S rRNA gene sequence analysis indeed confirmed that strain NEAU-GS84(T) belonged to the genus Herbidospora and was most closely related to Herbidospora yilanensis JCM 18062(T) (99.2% 16S rRNA gene sequence similarity) and Herbidospora galbida NEAU-GS14(T) (99.0%). The cell wall of strain NEAU-GS84(T) contained meso-diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates mainly contained glucose, madurose and ribose. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamin, phosphoglycolipids, two phosphatidylinositol mannosides and one unidentified phospholipid. The predominant menaquinone was MK-10(H-4). Major fatty acids were 10-methly C-17:0, C-17:0 and iso-C-16:0. These chemotaxonomic data substantiated the affiliation of strain NEAU-GS84(T) to the genus Herbidospora. The DNA G+C content was 70.7 mol%. The genome size of strain NEAU-GS84(T) is about 8.37 Mb and contained 41 cellulose-binding domain synthesis genes, 13 beta-glucosidase synthesis genes, 6 endoglucanase synthesis genes and 9 xylanase synthesis genes. Based on digital DNA-DNA hybridization and average nucleotide identity values, the new strain NEAU-GS84(T) could be differentiated from its closest relatives. Therefore, the strain represents a novel species of the genus Herbidospora, for which the name Herbidospora solisilvae sp. nov. is proposed. The type strain is NEAU-GS84(T) (= CCTCC AA 2018041(T) = JCM 33460(T)).

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