Vacuolar phosphate transporters account for variation in phosphate accumulation in Astragalus sinicus cultivars

文献类型： 外文期刊

第一作者： Guo, Zhenhui

作者： Guo, Zhenhui;Ruan, Wenyuan;Wu, Qingyu;Yi, Keke;Lyu, Yuhu

作者机构：

关键词： Astragalus sinicus; Green manure; Pi accumulation; Vacuolar Pi transporter

期刊名称：CROP JOURNAL （ 影响因子：4.407； 五年影响因子：5.687 ）

ISSN： 2095-5421

年卷期： 2021 年 9 卷 1 期

页码：

收录情况： SCI

摘要： Astragalus sinicus is a commonly used legume green manure that fixes atmospheric N-2 and accumulates mineral nutrients and organic substances that are beneficial to soils and subsequent crops. However, little is known about genotypic variation in, and molecular mechanisms of, Pi (phosphate) uptake and storage in A. sinicus. We recorded the morphological responses of six A. sinicus cultivars from four regions of China to external Pi application and measured their Pi accumulation. We identified full-length transcripts of Pi-signaling and Pi-homeostasis regulators by sequencing and measured the expression level of these genes by qRT-PCR. The major components in Pi signaling and Pi homeostasis were largely conserved between A. sinicus and the model species rice and Arabidopsis. Different A. sinicus varieties responded differently to low-phosphorus (P) stress, and their Pi accumulation was positively correlated with the expression of vacuolar Pi influx gene (SYG1/PHO81/XPR1-MAJOR FACILITATOR SUPERFAMILY (SPX-MFS)-TYPE PROTEIN) AsSPX-MFS2 and negatively correlated with the expression of the vacuolar Pi efflux gene (VACUOLAR Pi EFFLUX TRANSPORTER) AsVPE1. We identified key Pi-signaling and Pi-homeostasis regulators in A. sinicus. The expression of vacuolar Pi transporter genes could be used as an index to select A. sinicus accessions with high Pi accumulation. (C) 2020 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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