Micromonospora rubida sp. nov., a novel actinobacterium isolated from soil of Harbin

文献类型： 外文期刊

第一作者： Sun, Xiujun

作者： Sun, Xiujun;Qiu, Shiwen;Luo, Xianxian;Jin, Pinjiao;Zhao, Junwei;Wu, Xianyao;Yang, Jize;Wang, Xiangjing;Song, Jia;Xiang, Wensheng;Xiang, Wensheng

作者机构：

关键词： Genome; gyrB gene; Micromonospora rubida sp; nov; Polyphasic analysis; 16S rRNA gene

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.271； 五年影响因子：2.707 ）

ISSN： 0003-6072

年卷期： 2021 年 114 卷 6 期

页码：

收录情况： SCI

摘要： A novel actinobacterium, designated strain NEAU-HG-1(T), was isolated from soil collected from Harbin, Heilongjiang Province, Northeast China and characterised using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-HG-1(T) belonged to the genus Micromonospora, and shared high sequence similarities with Micromonospora auratinigra DSM 44815(T) (98.9%) and Micromonospora coerulea DSM 43143(T) (98.7%). Morphological and chemotaxonomic characteristics of the strain also supported its assignment to the genus Micromonospora. Cell wall contained meso-diaminopimelic acid and the whole-cell sugars were arabinose and xylose. The polar lipid contained diphosphatidylglycerol, phosphatidylethanolamine, glycolipid and phosphatidylinositol. The predominant menaquinones were MK-10(H-2), MK-10(H-4) and MK-10(H-6). The major fatty acids were C-17:0 cycle, iso-C-15:0, and iso-C-16:0. Furthermore, strain NEAU-HG-1(T) displayed a DNA-DNA relatedness of 33.8 +/- 2.2% with M. coerulea DSM 43143(T). The level of digital DNA-DNA hybridization between strain NEAU-HG-1(T) and M. auratinigra DSM 44815(T) was 27.2% (24.8-29.7%). The value was well below the criteria for species delineation of 70% for dDDH. Whole-genome average nucleotide identity analyses result also indicated that the isolate should be assigned to a new species under the genus Micromonospora. Therefore, it is concluded that strain NEAU-HG-1(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora rubida sp. nov. is proposed, with NEAU-HG-1(T) (= CGMCC 4.7479(T) = JCM 32386(T)) as the type strain.

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