Molecular cytogenetic characterization and fusarium head blight resistance of five wheat-Thinopyrum intermedium partial amphiploids

文献类型： 外文期刊

第一作者： Wang, Hui

作者： Wang, Hui;Cheng, Shuwei;Shi, Yue;Zhang, Shuxin;Yan, Wei;Jang, Bo;Qi, Xiaoyue;Li, Xinling;Zhang, Yanming;Song, Weifu;Yang, Xuefeng;Song, Qingjie;Friebe, Bernd

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关键词： Thinopyrum intermedium; Common wheat; Partial amphiploids; Fusarium head blight; In situ hybridization

期刊名称：MOLECULAR CYTOGENETICS （ 影响因子：2.009； 五年影响因子：1.714 ）

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年卷期： 2021 年 14 卷 1 期

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收录情况： SCI

摘要： Background Partial amphiploids created by crossing octoploid tritelytrigia(2n = 8x = 56, AABBDDEE) and Thinopyrum intermedium (2n = 6x = 42, StStJJJ(S)J(S)) are important intermediates in wheat breeding because of their resistance to major wheat diseases. We examined the chromosome compositions of five wheat-Th. intermedium partial amphiploids using GISH and multicolor-FISH. Results The result revealed that five lines had 10-14 J-genome chromosomes from Th. intermedium and 42 common wheat chromosomes, using the J-genomic DNA from Th. bessarabicum as GISH probe and the oligo probes pAs1-1, pAs1-3, AFA-4, (GAA) 10, and pSc119.2-1 as FISH probe. Five lines resembled their parent octoploid tritelytrigia (2n = 8x = 56, AABBDDEE) but had higher protein contents. Protein contents of two lines HS2-2 and HS2-5 were up to more than 20%. Evaluation of Fusarium head blight (FHB) resistance revealed that the percent of symptomatic spikelets (PSS) of these lines were below 30%. Lines HS2-2, HS2-4, HS2-5, and HS2-16 were less than 20% of PPS. Line HS2-5 with 14 J-genome chromosomes from Th. intermedium showed the best disease resistance, with PSS values of 10.8% and 16.6% in 2016 and 2017, respectively. Conclusions New wheat-Th. intermedium amphiploids with the J-genome chromosomes were identified and can be considered as a valuable source of FHB resistance in wheat breeding.

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