ZMK1 Is Involved in K+ Uptake and Regulated by Protein Kinase ZmCIPK23 in Zea mays

文献类型： 外文期刊

第一作者： Han, Wu

作者： Han, Wu;Ji, Yun;Wu, Wei;Cheng, Jin-Kui;Feng, Han-Qian;Wang, Yi;Feng, Han-Qian;Wang, Yi

作者机构：

关键词： Zea mays; ZmCIPK23; K+ uptake; K+ channel; ZMK1

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.402； 五年影响因子：5.207 ）

ISSN： 1664-462X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Potassium (K+) is one of essential mineral elements for plant growth and development. K+ channels, especially AKT1-like channels, play crucial roles in K+ uptake in plant roots. Maize is one of important crops; however, the K+ uptake mechanism in maize is little known. Here, we report the physiological functions of K+ channel ZMK1 in K+ uptake and homeostasis in maize. ZMK1 is a homolog of Arabidopsis AKT1 channel in maize, and mainly expressed in maize root. Yeast complementation experiments and electrophysiological characterization in Xenopus oocytes indicated that ZMK1 could mediate K+ uptake. ZMK1 rescued the low-K+-sensitive phenotype of akt1 mutant and enhanced K+ uptake in Arabidopsis. Overexpression of ZMK1 also significantly increased K+ uptake activity in maize, but led to an oversensitive phenotype. Similar to AKT1 regulation, the protein kinase ZmCIPK23 interacted with ZMK1 and phosphorylated the cytosolic region of ZMK1, activating ZMK1-mediated K+ uptake. ZmCIPK23 could also complement the low-K+-sensitive phenotype of Arabidopsis cipk23/lks1 mutant. These findings demonstrate that ZMK1 together with ZmCIPK23 plays important roles in K+ uptake and homeostasis in maize.

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