Comparative analysis of sialic acid alpha 2-3/6 galactose glycan-binding proteins in human saliva and serum

文献类型： 外文期刊

第一作者： Chen, Zhuo

作者： Chen, Zhuo;Chen, Wentian;Wang, Qinzhe;Qin, Yannan;Wang, Xilong;Ma, Tianran;Zhang, Peixin;Li, Xuetian;Ding, Li;Li, Zheng;Wang, Xiurong

作者机构：

关键词： SA alpha 2-3 Gal; SA alpha 2-6 Gal; Saliva; Serum; Influenza A virus (IAV); Glycan-binding proteins (GBPs)

期刊名称：JOURNAL OF MOLECULAR STRUCTURE （ 影响因子：2.463； 五年影响因子：2.121 ）

ISSN： 0022-2860

年卷期： 2021 年 1230 卷

页码：

收录情况： SCI

摘要： It has been discovered that SA alpha 2-6 Gal glycans prevailingly distribute in human upper respiratory tract while a few SA alpha 2-3 Gal glycans distribute in human lower respiratory tract. In this study, we aimed to separate and identify SA alpha 2-3/6 Gal glycan-binding proteins (GBPs) in human saliva and serum, since that these GBPs may possess anti-influenza A virus (anti-IAVs) protective effects by binding to receptor glycoconjugates earlier than virus hemagglutinin (HA). By using SA alpha 2-3/6 Gal glycan-magnetic particles complexes, a total of 116 GBPs (33 SA alpha 2-3 Gal GBPs, S3GBPs; 22 SA alpha 2-6 Gal GBPs, S6GBPs; and 61 SA alpha 23/6 Gal GBPs, SGBPs) in saliva and 144 GBPs (29 S3GBPs, 27 S6GBPs and 88 SGBPs) in serum were identified. Through emPAI analysis, for 61 saliva SGBPs, 16.39% showed higher extracting amount by SA alpha 23 Gal, and 57.38% showed higher extracting amount by SA alpha 2-6 Gal; for 88 serum SGBPs, only 2.27% showed higher extracting amount by SA alpha 2-3 Gal, and up to 89.77% showed higher extracting amount by SA alpha 2-6 Gal. A computational docking approach verified the docking between cystatin/apolipoprotein A-I and SA alpha 2-3/6 Gal. Our study may supply useful information to help understand the nature protective role of saliva, and the anti-IAVs double effects of proteins that simultaneously play sialoglycoprotein and SA alpha 2-3/6 Gal GBP roles. (C) 2021 Elsevier B.V. All rights reserved.

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