Pathogenic characteristics and infection-related genes of Metarhizium anisopliae FM-03 infecting Planococcus lilacinus

文献类型： 外文期刊

第一作者： Huang, Peng

作者： Huang, Peng;Yao, Jinai;Yu, Deyi;Lin, Yongwen

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关键词： Planococcus lilacinus; quarantine pest; Metarhizium anisopliae; pathogenicity; infection behavior; extracellular enzyme activity; infection‐ related genes; Hemiptera; Pseudococcidae; entomopathogenic fungus; sugar apple; Annona squamosa

期刊名称：ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA （ 影响因子：1.696； 五年影响因子：1.731 ）

ISSN： 0013-8703

年卷期： 2021 年 169 卷 5 期

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收录情况： SCI

摘要： Planococcus lilacinus (Cockerell) (Hemiptera: Pseudococcidae) is a global flower and fruit pest, and a severe infestation is currently seen in the sugar apple (Annona squamosa L., Annonaceae) orchards of Zhangzhou, Fujian Province, China. Biological control with entomopathogenic fungi is safe, effective, and long-lasting, and has potential for the control of mealybugs. In this study, we analyzed the pathogenic characteristics and infection-related genes of Metarhizium anisopliae (Metschn.) Sorokin FM-03, a strain discovered in the sugar apple orchards, against P. lilacinus, to evaluate its biocontrol potential and application prospects against mealybugs. The results showed that M. anisopliae FM-03 was highly pathogenic against P. lilacinus. At 10 days after infection, the median lethal concentration (LC50) of strain FM-03 was 1.45 x 10(5) spores ml(-1) and the cumulative corrected mortality was 93%, whereas the median lethal time (LT50) was 5.27 days for treatment with the highest spore concentration (10(8) spores ml(-1)). Planococcus lilacinus was primarily infected from the legs, abdominal segments, and body edges, which are sites with less wax. Infection became clearly visible 72 h post inoculation. Substantial changes occurred in activity of fungal enzymes, with peak values of 26 U ml(-1) for proteases and 17 U ml(-1) for lipases on day 5 post inoculation to enzyme inducing culture media. The activity of chitinases was relatively stable, with a peak value of 8 U ml(-1) on day 6 post inoculation. At 3 days post P. lilacinus infection, a total of 1 069 genes were differentially expressed, of which 137 were annotated as infection-related. Among the 136 upregulated genes, 10 were identified as potentially crucial for infection regulation. These 10 genes could be targeted for subsequent construction of genetically engineered FM-03 strains, driving the research and development of effective biocontrol agents for P. lilacinus.

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