Characterization of EstDR4, a Novel Cold-Adapted Insecticides-Metabolizing Esterase from Deinococcus radiodurans

文献类型： 外文期刊

第一作者： Zhang, Yage

作者： Zhang, Yage;Liu, Zhu;Zhang, Yage;Lu, Wei;Wang, Jin;Chen, Ming;Zhang, Wei;Lin, Min;Zhou, Zhengfu

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关键词： EstDR4; esterase; D; radiodurans; cold adapted; insecticide degradation

期刊名称：APPLIED SCIENCES-BASEL （ 影响因子：2.474； 五年影响因子：2.458 ）

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年卷期： 2021 年 11 卷 4 期

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收录情况： SCI

摘要： Cold-adapted esterases are attracting increasing attention owing to their prospective use in biotechnology. In this study, a novel cold-adapted family esterase EstDR4 was identified and obtained from extremophile Deinococcus radiodurans (D. radiodurans). EstDR4 displayed significant substrate preference towards short and medium chain monoesters (C2-C12). It also showed regioselectivity, enantioselectivity and degradation effects on four insecticides. The optimum temperature and pH for EstDR4 activity were 30 degrees C and pH 8, respectively. Additionally, EstDR4 exhibited relatively high catalytic activity at 0 degrees C and high stability from 10-40 degrees C, with over 80% of its initial activity retained after 1 h of incubation. Moreover, EstDR4 activity was stimulated by Tween 80 and Triton X-100, and inhibited by metal ions such as Co2+, Cu2+ and Zn2+ and several organic solvents. Thus, this enzyme shows development potential for many industrial biotechnological applications, including the manufacture of thermolabile pharmaceutical products, cold-wash detergents and insecticide biodegradation.

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