A practical and fast isolation of 12 cyclolinopeptides (linusorbs) from flaxseed oil via preparative HPLC with phenyl-hexyl column
文献类型: 外文期刊
第一作者: Liu, Xue
作者: Liu, Xue;Cai, Zi-zhe;Lee, Wan Jun;Lu, Xuan-xuan;Reaney, Martin J. T.;Li, Ying;Zhang, Ning;Wang, Yong;Reaney, Martin J. T.;Zhang, Jian-ping
作者机构:
关键词: Flaxseed oil; Cyclolinopeptide; Phenyl-hexyl column; Preparative HPLC
期刊名称:FOOD CHEMISTRY ( 影响因子:6.306; 五年影响因子:6.219 )
ISSN: 0308-8146
年卷期: 2021 年 351 卷
页码:
收录情况: SCI
摘要: Linusorbs, known as cyclolinopeptides, are a group of cyclic hydrophobic peptides derived from flaxseed oil with various health benefits. However, the current research efforts on both the biological activities and antioxidant capacities of linusorbs are limited because of existing issues with their purification and characterization. A practical method based on preparative HPLC for isolating 12 linusorbs simultaneously was developed and factors such as the solvent selection, gradient elution program, flow rate, loaded mass, and loading concentration, were optimized. The optimum conditions were an initial acetonitrile (ACN) to water ratio of 40%, final ACN ratio of 80%, eluting time of 21 min, a flow rate of 16 mL/min, sample load of 12.5 mg, and concentration of 80 mg/mL (in methanol). The 12 linusorbs obtained were verified using off-line MS/MS, recording purities of above 95.5%. The method could serve as a practical and fast isolation method enabling further investigation of minor linusorbs.
分类号:
- 相关文献
作者其他论文 更多>>
-
Research on Positioning and Navigation System of Greenhouse Mobile Robot Based on Multi-Sensor Fusion
作者:Cheng, Bo;Li, Xiaoyue;Zhang, Ning;Song, Weitang;He, Xueying;Wu, Huarui
关键词:agricultural greenhouse; navigation robot; multi-sensor fusion; ultra-wideband; inertial measurement unit; odometry; rangefinder
-
Effector Protein Serine Carboxypeptidase FgSCP Is Essential for Full Virulence in Fusarium graminearum and Is Involved in Modulating Plant Immune Responses
作者:Liu, Kouhan;Wang, Xintong;Li, Ying;Shi, Yifeng;Ren, Yanyan;Wang, Aolin;Cheng, Peng;Wang, Baotong;Qi, Yuzhe
关键词:effector; Fusarium graminearum; serine carboxypeptidases; virulence
-
Nitrogen addition alters aboveground C:N:P stoichiometry of plants but not for belowground in an Inner Mongolia grassland
作者:Wang, Ziqi;An, Yixin;Li, Ying;Yu, Qiang;Wang, Jie;Wang, Xu;Wu, Honghui;Yang, Tian;Zhang, Yunlong;Bian, Jianlin;Ren, Haiyan;Lkhagva, Ariuntsetseg
关键词:nitrogen deposition; C:N:P stoichiometry; grassland ecosystem; community level; belowground
-
Unraveling the regulatory network of miRNA expression in Potato Y virus-infected of Nicotiana benthamiana using integrated small RNA and transcriptome sequencing
作者:Song, Hongping;Zhang, Songbai;Gao, Xinwen;Song, Liyun;Jiao, Yubing;Shen, Lili;Yang, Jinguang;Li, Ying;Li, Changquan;Shang, Jun;Wang, Hui
关键词:high-throughput sequencing; miRNA; Potato virus Y; miRNA-mRNA network; viral infection
-
Gibberellins involved in fruit ripening and softening by mediating multiple hormonal signals in tomato
作者:Wu, Mengbo;Li, Ying;Zhu, Yunqi;Su, Dan;Zhang, Yaoxin;Deng, Heng;Liu, Mingchun;Liu, Kaidong;Li, Honghai;Wang, Yikui
关键词:
-
Large-scale gene expression alterations introduced by structural variation drive morphotype diversification in Brassica oleracea
作者:Li, Xing;Wang, Yong;Cai, Chengcheng;Ji, Jialei;Han, Fengqing;Zhang, Lei;Chen, Shumin;Zhang, Lingkui;Yang, Yinqing;Tang, Qi;Wang, Xuelin;Yang, Limei;Zhuang, Mu;Zhang, Kang;Lv, Honghao;Zhang, Yangyong;Cheng, Feng;Cai, Chengcheng;Bucher, Johan;Bonnema, Guusje
关键词:
-
GhBRX.1, GhBRX.2, and GhBRX4.3 improve resistance to salt and cold stress in upland cotton
作者:Wei, Wei;Ju, Jisheng;Zhang, Xueli;Ling, Pingjie;Luo, Jin;Li, Ying;Xu, Wenjuan;Su, Junji;Wang, Caixiang;Su, Junji;Zhang, Xianliang;Zhang, Xianliang
关键词:BREVIS RADIX; salt stress; cold stress; virus-induced gene silencing (VIGS); upland cotton