Genetic Diversity of Castor Bean (Ricinus communis L.) Revealed by ISSR and RAPD Markers

文献类型： 外文期刊

第一作者： Kim, HyokChol

作者： Kim, HyokChol;Lei, Pei;Wang, Aizhi;Liu, Shuo;Meng, Fanjuan;Zhao, Yong;Huang, Fenglan;Zhao, Yong;Huang, Fenglan;Yu, Zhenliang;Zhu, Guoli;He, Zhibiao;Tan, Deyun;Wang, Hongwei

作者机构：

关键词： Ricinus communis L.; genetic diversity; inter simple sequence repeat (ISSR); random amplified polymorphic DNA (RAPD)

期刊名称：AGRONOMY-BASEL （ 影响因子：2.603； ）

ISSN：

年卷期： 2021 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Castor (Ricinus communis L.), known as castor oil plant or castor bean, is a non-edible oilseed crop. In the present study, the genetic diversity among 54 samples (3 wild and 51 cultivated) collected worldwide was evaluated using inter-simple sequence repeats (ISSRs) and random amplified polymorphic DNA (RAPD) markers. A total of 9 ISSR primers produced 83 high-resolution bands with 61 (74.53%) as polymorphic. The percentage of polymorphic bands per primer and the genetic similarity coefficient ranged from 54.55% (UBC-836) to 100% (UBC-808) and from 0.74 to 0.96, respectively. A total of 11 out of 20 RAPD primers amplified unique polymorphic products with an average percentage of polymorphic bands of 60.98% (56 polymorphic bands out of a total of 90 bands obtained). The percentage of polymorphic bands per primer ranged from 25% (OPA-02 and B7) to 90.91% (B21) with the genetic similarity coefficient ranging from 0.73 to 0.98. The unweighted pair group method with arithmetic averages (UPGMA) dendrogram using two molecular markers divided 54 castor genotypes into three groups. Furthermore, based on morphological data, all 54 castor varieties were grouped into three main clusters. The genetic diversity analysis based on two molecular makers showed that most varieties from China were closely related to each other with three varieties (GUANGDONGwild, ZHEJIANGWild, and HANNANWild) belonging to a wild group separated from most of the cultivated castor samples from China, India, France, and Jordan. These results suggested that the cultivated castor contains a narrow genetic base. Accordingly, we recommend that wild castor genetic resources be introduced for breeding novel castor varieties. Furthermore, the Vietnam, Malaysia, Indonesia, and Nigeria accessions were clustered into the same group. The results of principal coordinate analysis (PCoA) and UPGMA cluster analysis were consistent with each other. The findings of this study are important for future breeding studies of castor.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序