The Identification of Mutation in BMP15 Gene Associated with Litter Size in Xinjiang Cele Black Sheep

文献类型： 外文期刊

第一作者： Niu, Zhi-gang

作者： Niu, Zhi-gang;Ding, Yu-gong;Tang, Sen;Shi, Hong-cai;Qin, Jin;Jiang, Yao;Ding, Xiang-Dong

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关键词： Xinjiang Cele black sheep; bone morphogenetic protein 15 gene; mutation; fecundity; litter size

期刊名称：ANIMALS （ 影响因子：2.323； ）

ISSN： 2076-2615

年卷期： 2021 年 11 卷 3 期

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收录情况： SCI

摘要： Simple Summary The gene for Bone Morphogenetic Protein 15 has multiple single-nucleotide polymorphism sites related to fertility in sheep. To increase fertility, a better understanding of the regulation of Bone Morphogenetic Protein 15 in sheep is essential. This work describes the phenotype and molecular characteristics of a mutation identified in the Bone Morphogenetic Protein 15 gene of Cele black sheep in Xinjiang, China. This mutation may affect the fertility of sheep, which is very useful for breeding purposes. The Bone Morphogenetic Protein 15 (BMP15) gene is known to have multiple single-nucleotide polymorphism sites associated with sheep fecundity. This study used gene sequence analysis and mutation detection assays for BMP15 by using 205 blood samples of ewes with known lambing records. Sequence analysis showed that mutation B1 missed the CTT base in exon 1 at positions 28-30, leading to a leucine deletion in the BMP15 protein. Litter size of ewes differed significantly between BB and B+ genotypes of B1 (p < 0.05); however, the differences between wild genotype (++) and homozygous (BB) or wild genotype (++) and heterozygous (B+) were not significant (p > 0.05). Another mutation, T755C, is a T-to-C base change at position 755 of exon 2, resulting in leucine replacement by proline at this position of the BMP15 protein (p.L252P). Two genotypes were identified in the flock: heterozygous (E+) and wild-type genotype (++). Ewes with heterozygous (E+) p.L252P had significantly larger litter sizes than those with the wild-type genotype (p < 0.05). Comprehensive analysis suggests that p.L252P is a mutation that affects fecundity in Cele black sheep.

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