Five TPSs are responsible for volatile terpenoid biosynthesis in Albizia julibrissin

文献类型： 外文期刊

第一作者： Liu, Guanhua

作者： Liu, Guanhua;Yang, Mei;Fu, Jianyu;Liu, Guanhua;Yang, Mei;Liu, Guanhua;Yang, Mei;Fu, Jianyu;Liu, Guanhua;Yang, Xuemin;Ma, Xiaoying

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关键词： Volatiles; Flower scent; Terpene; Terpene synthases; A. julibrissin

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.013； 五年影响因子：3.615 ）

ISSN： 0176-1617

年卷期： 2021 年 258 卷

页码：

收录情况： SCI

摘要： Silk tree, Albizia julibrissin Duraz, is an old ornamental plant and extensively cultivated in Asia. Previous works have discovered that the terpenoids were the dominating compounds in the floral VOC of A. julibrissin, however the biosynthesis of these terpenoids was poorly understood so far. Here, 11 terpene synthase genes (TPSs) were identified by transcriptome sequencing that fell into TPS-a, TPS-b and TPS-g subfamilies. The enzymatic activity tests showed that five genes were functional: AjTPS2 was a sesquiterpene synthase and produced alpha-farnesene and (Z, E)-beta-farnesene; AjTPS5 was able to catalyze the formation of five monoterpenes and nine sesquiterpenes; AjTPS7, AjTPS9 and AjTPS10 were dedicated monoterpene synthases, as AjTPS7 and AjTPS10 formed the single product beta-ocimene and linalool, respectively, and AjTPS9 produced gamma-terpinene with other three monoterpenes. More importantly, the main catalytic products of the characterized AjTPSs were consistent with the terpenoids observed in A. julibrissin volatiles. Combining terpene chemistry, TPSs biochemical activities and gene expression analysis, we demonstrate that AjTPS2, AjTPS5, AjTPS7, AjTPS9 and AjTPS10 are responsible for the volatile terpenoids biosynthesis in A. julibrissin.

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