Wheat Varietal Response to Tilletia controversa J. G. Kuhn Using qRT-PCR and Laser Confocal Microscopy

文献类型： 外文期刊

第一作者： Chen, Delai

作者： Chen, Delai;Liu, Changzhong;Chen, Delai;Muhae-Ud-Din, Ghulam;Liu, Taiguo;Chen, Wanquan;Gao, Li

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关键词： wheat dwarf bunt; TaPR genes; tapetum; pollen grain; laser confocal microscopy

期刊名称：GENES （ 影响因子：3.759； 五年影响因子：3.822 ）

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年卷期： 2021 年 12 卷 3 期

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收录情况： SCI

摘要： Tilletia controversa J. G. Kuhn is a causal organism of dwarf bunt in wheat. Understanding the interaction of wheat and T. controversa is of practical and scientific importance for disease control. In this study, the relative expression of TaLHY and TaPR-4 and TaPR-5 genes was higher in a resistant (Yinong 18) and moderately resistant (Pin 9928) cultivars rather than susceptible (Dongxuan 3) cultivar at 72 h post inoculation (hpi) with T. controversa. Similarly, the expression of defensin, TaPR-2 and TaPR-10 genes was observed higher in resistant and moderately resistant cultivars after exogenous application of phytohormones, including methyl jasmonate, salicylic acid, and abscisic acid. Laser confocal microscopy was used to track the fungal hyphae in the roots, leaves, and tapetum cells, which of susceptible cultivar were infected harshly by T. controversa than moderately resistant and resistant cultivars. There were no fungal hyphae in tapetum cells in susceptible cultivar after methyl jasmonate, salicylic acid and abscisic acid treatments. Moreover, after T. controversa infection, the pollen germination was of 80.06, 58.73, and 0.67% in resistant, moderately resistant and susceptible cultivars, respectively. The above results suggested that the use using of resistant cultivar is a good option against the dwarf bunt disease.

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