Preparation of Active Chitooligosaccharides with a Novel Chitosanase AqCoA and Their Application in Fungal Disease Protection

文献类型： 外文期刊

第一作者： Wang, Yanxin

作者： Wang, Yanxin;Xia, Chengyao;Fan, Qiwen;Li, Xu;Lan, Zejun;Shi, Guolong;Li, Zhoukun;Cui, Zhongli;Li, Ding;Liu, Muxing;Dong, Weiliang

作者机构：

关键词： chitosanase; chitosan oligosaccharides; controllable hydrolysis; antifungal properties; plant immunity activation; biocontrol agent

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：4.192； 五年影响因子：4.289 ）

ISSN： 0021-8561

年卷期： 2021 年 69 卷 11 期

页码：

收录情况： SCI

摘要： Enzymes that degrade fungal cell walls and the resulting oligosaccharides are promising weapons to combat plant fungal disease. In this study, we identified a novel endo-chitosanase, AqCoA, from Aquabacterium sp. A7-Y. The enzyme showed a specific activity of 18 U/mg toward 95% deacetylated chitosan at pH 5.0 and 40 degrees C. AqCoA also showed activity toward sodium carboxymethylcellulose, indicating substrate promiscuity. AqCoA hydrolyzed chitosan into chitooligosaccharides (CoA-COSs) with degrees of polymerization (DPs) of 3-5 but showed no activity toward CoA-COSs with DPs <6, indicating an endo-type activity. At 2.5 mu g/mL, AqCoA inhibited appressorium formation of Magnaporthe oryzae; the produced CoA-COSs also inhibited the growth of M. oryzae and Fusarium oxysporum. Furthermore, CoA-COSs acted as immune elicitors in rice by inducing the reactive oxygen species burst and the expression of defense genes. These results demonstrated that AqCoA and its resulting CoA-COSs might be effective tools for protecting plants against pathogenic fungi.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序